Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Uridylate kinase

Gene

pyrH

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.UniRule annotation

Catalytic activityi

ATP + UMP = ADP + UDP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP. Inhibited by UTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55UMP; via amide nitrogenUniRule annotation1
Binding sitei56ATP; via amide nitrogenUniRule annotation1
Binding sitei60ATPUniRule annotation1
Binding sitei75UMPUniRule annotation1
Binding sitei164ATPUniRule annotation1
Binding sitei170ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei173ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 16ATPUniRule annotation4
Nucleotide bindingi136 – 143UMPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processPyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinaseUniRule annotation (EC:2.7.4.22UniRule annotation)
Short name:
UKUniRule annotation
Alternative name(s):
Uridine monophosphate kinaseUniRule annotation
Short name:
UMP kinaseUniRule annotation
Short name:
UMPKUniRule annotation
Gene namesi
Name:pyrHUniRule annotation
Synonyms:smbA
Ordered Locus Names:SA1101
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001438831 – 240Uridylate kinaseAdd BLAST240

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP65936
SMRiP65936
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 26Involved in allosteric activation by GTPUniRule annotation6

Sequence similaritiesi

Belongs to the UMP kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000047187
KOiK09903
OMAiPIIVFDM

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_01220_B PyrH_B, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR011817 Uridylate_kinase
IPR015963 Uridylate_kinase_bac
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF005650 Uridylate_kin, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR02075 pyrH_bact, 1 hit

Sequencei

Sequence statusi: Complete.

P65936-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQISKYKRV VLKLSGEALA GEKGFGINPV IIKSVAEQVA EVAKMDCEIA
60 70 80 90 100
VIVGGGNIWR GKTGSDLGMD RGTADYMGML ATVMNALALQ DSLEQLDCDT
110 120 130 140 150
RVLTSIEMKQ VAEPYIRRRA IRHLEKKRVV IFAAGIGNPY FSTDTTAALR
160 170 180 190 200
AAEVEADVIL MGKNNVDGVY SADPKVNKDA VKYEHLTHIQ MLQEGLQVMD
210 220 230 240
STASSFCMDN NIPLTVFSIM EEGNIKRAVM GEKIGTLITK
Length:240
Mass (Da):26,145
Last modified:October 11, 2004 - v1
Checksum:iFD4099DEC5F87C11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA Translation: BAB42353.1
PIRiE89899
RefSeqiWP_000057330.1, NC_002745.2

Genome annotation databases

EnsemblBacteriaiBAB42353; BAB42353; BAB42353
KEGGisau:SA1101

Similar proteinsi

Entry informationi

Entry nameiPYRH_STAAN
AccessioniPrimary (citable) accession number: P65936
Secondary accession number(s): P59006, Q99UL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health