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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei94 – 9415-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei98 – 9815-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei100 – 10015-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei124 – 1241OrotateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1223-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:SAV1205
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203Orotate phosphoribosyltransferasePRO_0000110737Add
BLAST

Proteomic databases

PaxDbiP65916.

2D gel databases

World-2DPAGE0002:P65916.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV1205.

Structurei

3D structure databases

ProteinModelPortaliP65916.
SMRiP65916. Positions 1-203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni120 – 12895-phosphoribose 1-diphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.
OrthoDBiEOG6C2WDW.
PhylomeDBiP65916.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEIAKSLL DIEAVTLSPN DLYTWSSGIK SPIYCDNRVT LGYPLVRGAI
60 70 80 90 100
RDGLINLIKE HFPEVEVISG TATAGIPHAA FIAEKLKLPM NYVRSSNKSH
110 120 130 140 150
GKQNQIEGAK SEGKKVVVIE DLISTGGSSV TAVEALKQAG AEVLGVVAIF
160 170 180 190 200
TYGLKKADDT FSNIQLPFYT LSDYNELIEV AENEGKISSE DIQTLVEWRD

NLA
Length:203
Mass (Da):22,057
Last modified:October 11, 2004 - v1
Checksum:i11F5127DE1E5D8E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57367.1.
RefSeqiWP_001040248.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57367; BAB57367; SAV1205.
KEGGisav:SAV1205.
PATRICi19563121. VBIStaAur52173_1238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57367.1.
RefSeqiWP_001040248.1. NC_002758.2.

3D structure databases

ProteinModelPortaliP65916.
SMRiP65916. Positions 1-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1205.

2D gel databases

World-2DPAGE0002:P65916.

Proteomic databases

PaxDbiP65916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB57367; BAB57367; SAV1205.
KEGGisav:SAV1205.
PATRICi19563121. VBIStaAur52173_1238.

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.
OrthoDBiEOG6C2WDW.
PhylomeDBiP65916.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciSAUR158878:GJJ5-1223-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiPYRE_STAAM
AccessioniPrimary (citable) accession number: P65916
Secondary accession number(s): Q99UR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 16, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.