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P65898 (PURK_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase
Gene names
Name:purK
Ordered Locus Names:Rv3276c, MT3376
ORF Names:MTCY71.16c
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.

Catalytic activity

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.

Subunit structure

Homodimer By similarity.

Miscellaneous

Was identified as a high-confidence drug target.

Sequence similarities

Belongs to the purK/purT family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429N5-carboxyaminoimidazole ribonucleotide synthase
PRO_0000075000

Regions

Domain121 – 310190ATP-grasp
Nucleotide binding194 – 1974ATP By similarity
Nucleotide binding280 – 2812ATP By similarity

Sites

Binding site1171ATP By similarity
Binding site1571ATP By similarity
Binding site2021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P65898 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 1A80B4CCAB8CB13E

FASTA42945,695
        10         20         30         40         50         60 
MMAVASSRTP AVTSFIAPLV AMVGGGQLAR MTHQAAIALG QNLRVLVTSA DDPAAQVTPN 

        70         80         90        100        110        120 
VVIGSHTDLA ALRRVAAGAD VLTFDHEHVP NELLEKLVAD GVNVAPSPQA LVHAQDKLVM 

       130        140        150        160        170        180 
RQRLAAAGVA VPRYAGIKDP DEIDVFAARV DAPIVVKAVR GGYDGRGVRM ARDVADARDF 

       190        200        210        220        230        240 
ARECLADGVA VLVEERVDLR RELSALVARS PFGQGAAWPV VQTVQRDGTC VLVIAPAPAL 

       250        260        270        280        290        300 
PDDLATAAQR LALQLADELG VVGVLAVELF ETTDGALLVN ELAMRPHNSG HWTIDGARTS 

       310        320        330        340        350        360 
QFEQHLRAVL DYPLGDSDAV VPVTVMANVL GAAQPPAMSV DERLHHLFAR MPDARVHLYG 

       370        380        390        400        410        420 
KAERPGRKVG HINFLGSDVA QLCERAELAA HWLSHGRWTD GWDPHRASDD AVGVPPACGG 


RSDEEERRL

« Hide

References

[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis."
Raman K., Yeturu K., Chandra N.
BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract]
Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842582 Genomic DNA. Translation: CAB07079.1.
AE000516 Genomic DNA. Translation: AAK47717.1.
PIRE70979.
RefSeqNP_217793.1. NC_000962.2.
NP_337903.1. NC_002755.2.

3D structure databases

ProteinModelPortalP65898.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000002357; EBMYCP00000002357; EBMYCG00000002355.
EBMYCT00000069902; EBMYCP00000067961; EBMYCG00000069897.
GeneID888720.
922458.
GenomeReviewsGene locus MT3376 in contig AE000516_GR.
Gene locus Rv3276c in contig AL123456_GR.
KEGGmtc:MT3376.
mtu:Rv3276c.
PATRIC18129174. VBIMycTub22151_3683.
TIGRMT3376.

Organism-specific databases

TubercuListRv3276c.

Phylogenomic databases

GeneTreeEBGT00050000015722.
HOGENOMHBG516369.
OMAGRKMGHF.
PhylomeDBP65898.
ProtClustDBPRK06019.

Family and domain databases

InterProIPR005875. AIR_COase_ATPase-su.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01589.
PfamPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR01161. PurK. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePURK_MYCTU
AccessionPrimary (citable) accession number: P65898
Secondary accession number(s): P96881
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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