P65897 (PUR3_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART

Gene names

Name:	purN
Ordered Locus Names:	SAV1072

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Phosphoribosylglycinamide formyltransferase		PRO_0000074946
Regions
	Region	12 – 14	3	5'-phosphoribosylglycinamide binding By similarity
	Region	91 – 94	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	110	1	Proton donor By similarity
	Binding site	66	1	10-formyltetrahydrofolate By similarity
	Binding site	108	1	10-formyltetrahydrofolate By similarity
	Binding site	146	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	175	1	5'-phosphoribosylglycinamide By similarity
	Site	146	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65897 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: F0364618F275FA30	FASTA	188	21,166
	10 20 30 40 50 60 MVKIAIFASG SGSNFENIVE HVESGKLENI EVTALYTDHQ NAFCIDRAKK HDIPVYINEP 70 80 90 100 110 120 KQFDSKAAYE QHLVTLLNKD KVEWIILAGY MRLIGPDLLA SFEGKILNIH PSLLPKYKGI 130 140 150 160 170 180 DAIGQAYHSG DTITGSTVHY VDCGMDTGEI IEQRQCDIRP DDSKEQLEEK VKKLEYELYP SVIAKIVK

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB57234.1.
RefSeq	NP_371596.1. NC_002758.2.
3D structure databases
ProteinModelPortal	P65897.
ModBase	Search...
Protein-protein interaction databases
STRING	P65897.
2D gel databases
World-2DPAGE	0002:P65897.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000006422; EBSTAP00000006240; EBSTAG00000006421.
GeneID	1121049.
GenomeReviews	Gene locus SAV1072 in contig BA000017_GR.
KEGG	sav:SAV1072.
PATRIC	19562843. VBIStaAur52173_1100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0299.
GeneTree	EBGT00050000024715.
HOGENOM	HBG649382.
OMA	GSNMQAI.
PhylomeDB	P65897.
ProtClustDB	CLSK885118.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV1072-MONOMER.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K11175.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_STAAM

Accession

Primary (citable) accession number: P65897
Secondary accession number(s): Q99V25

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program