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P65895

- PUR2_STAAM

UniProt

P65895 - PUR2_STAAM

Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi281 – 2811Magnesium or manganeseUniRule annotation
    Metal bindingi283 – 2831Magnesium or manganeseUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi134 – 19158ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. manganese ion binding Source: UniProtKB-HAMAP
    4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAUR158878:GJJ5-1093-MONOMER.
    UniPathwayiUPA00074; UER00125.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
    Alternative name(s):
    GARSUniRule annotation
    Glycinamide ribonucleotide synthetaseUniRule annotation
    Phosphoribosylglycinamide synthetaseUniRule annotation
    Gene namesi
    Name:purDUniRule annotation
    Ordered Locus Names:SAV1074
    OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
    Taxonomic identifieri158878 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002481: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415Phosphoribosylamine--glycine ligasePRO_0000151479Add
    BLAST

    Proteomic databases

    PRIDEiP65895.

    2D gel databases

    World-2DPAGE0002:P65895.

    Interactioni

    Protein-protein interaction databases

    STRINGi158878.SAV1074.

    Structurei

    3D structure databases

    ProteinModelPortaliP65895.
    SMRiP65895. Positions 1-412.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 311204ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GARS family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0151.
    HOGENOMiHOG000033463.
    KOiK01945.
    OMAiYKGFLYA.
    OrthoDBiEOG69SKD1.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPiMF_00138. GARS.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P65895-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNVLVIGAGG REHALAYKLN QSNLVKQEFV IPGNEAMTPI AEVHTEISES    50
    NHQGILDFAK QQNVDWVVIG PEQPLIDGLA DILRANGFKV FGPNKQAAQI 100
    EGSKLFAKKI MKKYNIPTAD YKEVERKKDA LTYIENCELP VVVKKDGLAA 150
    GKGVIIADTI EAARSAIEIM YGDEEEGTVV FETFLEGEEF SLMTFVNGDL 200
    AVPFDCIAQD HKRAFDHDEG PNTGGMGAYC PVPHISDDVL KLTNETIAQP 250
    IAKAMLNEGY QFFGVLYIGA ILTKDGPKVI EFNARFGDPE AQVLLSRMES 300
    DLMQHIIDLD EGKRTEFKWK NESIVGVMLA SKGYPDAYEK GHKVSGFDLN 350
    ENYFVSGLKK QGDTFVTSGG RVILAIGKGD NVQDAQRDAY EKVSQIQSDH 400
    LFYRHDIANK ALQLK 415
    Length:415
    Mass (Da):45,836
    Last modified:October 11, 2004 - v1
    Checksum:i2F4A9770D6AA2523
    GO

    Sequence cautioni

    The sequence BAB57236.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57236.1. Different initiation.
    RefSeqiNP_371598.1. NC_002758.2.

    Genome annotation databases

    EnsemblBacteriaiBAB57236; BAB57236; SAV1074.
    GeneIDi1121051.
    KEGGisav:SAV1074.
    PATRICi19562847. VBIStaAur52173_1102.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57236.1 . Different initiation.
    RefSeqi NP_371598.1. NC_002758.2.

    3D structure databases

    ProteinModelPortali P65895.
    SMRi P65895. Positions 1-412.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158878.SAV1074.

    2D gel databases

    World-2DPAGE 0002:P65895.

    Proteomic databases

    PRIDEi P65895.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB57236 ; BAB57236 ; SAV1074 .
    GeneIDi 1121051.
    KEGGi sav:SAV1074.
    PATRICi 19562847. VBIStaAur52173_1102.

    Phylogenomic databases

    eggNOGi COG0151.
    HOGENOMi HOG000033463.
    KOi K01945.
    OMAi YKGFLYA.
    OrthoDBi EOG69SKD1.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    BioCyci SAUR158878:GJJ5-1093-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPi MF_00138. GARS.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mu50 / ATCC 700699.

    Entry informationi

    Entry nameiPUR2_STAAM
    AccessioniPrimary (citable) accession number: P65895
    Secondary accession number(s): Q99V23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3