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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi282Magnesium or manganeseUniRule annotation1
Metal bindingi284Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 193ATPUniRule annotationAdd BLAST57

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-808-MONOMER
UniPathwayiUPA00074; UER00125

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:BQ2027_MB0795
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514651 – 422Phosphoribosylamine--glycine ligaseAdd BLAST422

Structurei

3D structure databases

ProteinModelPortaliP65894
SMRiP65894
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 312ATP-graspUniRule annotationAdd BLAST206

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000033463
OMAiKATVCKY

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

P65894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLVIGSGA REHALLLALG KDPQVSGLIV APGNAGTARI AEQHDVDITS
60 70 80 90 100
AEAVVALARE VGADMVVIGP EVPLVLGVAD AVRAAGIVCF GPGKDAARIE
110 120 130 140 150
GSKAFAKDVM AAAGVRTANS EIVDSPAHLD AALDRFGPPA GDPAWVVKDD
160 170 180 190 200
RLAAGKGVVV TADRDVARAH GAALLEAGHP VLLESYLDGP EVSLFCVVDR
210 220 230 240 250
TVVVPLLPAQ DFKRVGEDDT GLNTGGMGAY APLPWLPDNI YREVVSRIVE
260 270 280 290 300
PVAAELVRRG SSFCGLLYVG LAITARGPAV VEFNCRFGDP ETQAVLALLE
310 320 330 340 350
SPLGQLLHAA ATGKLADFGE LRWRDGVAVT VVLAAENYPG RPRVGDVVVG
360 370 380 390 400
SEAEGVLHAG TTRRDDGAIV SSGGRVLSVV GTGADLSAAR AHAYEILSSI
410 420
RLPGGHFRSD IGLRAAEGKI SV
Length:422
Mass (Da):43,509
Last modified:October 11, 2004 - v1
Checksum:i1FB82706EBEB19A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIT99394.1
RefSeqiNP_854453.1, NC_002945.3
WP_003898580.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO42040; CDO42040; Mb0795
PATRICifig|233413.5.peg.865

Similar proteinsi

Entry informationi

Entry nameiPUR2_MYCBO
AccessioniPrimary (citable) accession number: P65894
Secondary accession number(s): A0A1R3XWF7, P71827, X2BG60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health