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P65893 (PUR2_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Rv0772, MT0796
ORF Names:MTCY369.17
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Phosphoribosylamine--glycine ligase HAMAP MF_00138
PRO_0000151464

Regions

Domain107 – 312206ATP-grasp
Nucleotide binding137 – 19357ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
P65893 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 1FB82706EBEB19A1

FASTA42243,509
        10         20         30         40         50         60 
MRVLVIGSGA REHALLLALG KDPQVSGLIV APGNAGTARI AEQHDVDITS AEAVVALARE 

        70         80         90        100        110        120 
VGADMVVIGP EVPLVLGVAD AVRAAGIVCF GPGKDAARIE GSKAFAKDVM AAAGVRTANS 

       130        140        150        160        170        180 
EIVDSPAHLD AALDRFGPPA GDPAWVVKDD RLAAGKGVVV TADRDVARAH GAALLEAGHP 

       190        200        210        220        230        240 
VLLESYLDGP EVSLFCVVDR TVVVPLLPAQ DFKRVGEDDT GLNTGGMGAY APLPWLPDNI 

       250        260        270        280        290        300 
YREVVSRIVE PVAAELVRRG SSFCGLLYVG LAITARGPAV VEFNCRFGDP ETQAVLALLE 

       310        320        330        340        350        360 
SPLGQLLHAA ATGKLADFGE LRWRDGVAVT VVLAAENYPG RPRVGDVVVG SEAEGVLHAG 

       370        380        390        400        410        420 
TTRRDDGAIV SSGGRVLSVV GTGADLSAAR AHAYEILSSI RLPGGHFRSD IGLRAAEGKI 


SV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842574 Genomic DNA. Translation: CAB02384.1.
AE000516 Genomic DNA. Translation: AAK45038.1.
PIRG70707.
RefSeqNP_215286.1. NC_000962.2.
NP_335224.1. NC_002755.2.

3D structure databases

ProteinModelPortalP65893.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000002368; EBMYCP00000002368; EBMYCG00000002366.
EBMYCT00000069266; EBMYCP00000067325; EBMYCG00000069261.
GeneID888873.
926099.
GenomeReviewsGene locus MT0796 in contig AE000516_GR.
Gene locus Rv0772 in contig AL123456_GR.
KEGGmtc:MT0796.
mtu:Rv0772.
PATRIC18123492. VBIMycTub22151_0874.
TIGRMT0796.

Organism-specific databases

TubercuListRv0772.

Phylogenomic databases

GeneTreeEBGT00050000015554.
HOGENOMHBG404060.
OMAMGAYTPL.
PhylomeDBP65893.
ProtClustDBPRK00885.

Family and domain databases

HAMAPMF_00138. GARS.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.90.600.10. Gars. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01945.
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR00877. PurD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_MYCTU
AccessionPrimary (citable) accession number: P65893
Secondary accession number(s): P71827
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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