Reviewed,
UniProtKB/Swiss-Prot P65893 (PUR2_MYCTU)
Last modified
November 3, 2009.
Version 39.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Phosphoribosylamine--glycine ligase EC=6.3.4.13 Alternative name(s): GARS Glycinamide ribonucleotide synthetase Phosphoribosylglycinamide synthetase | ||||||
| Gene names |
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| Organism | Mycobacterium tuberculosis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1773 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex |
Protein attributes
| Sequence length | 422 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138 |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. |
| Pathway | Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138 |
| Sequence similarities | Belongs to the GARS family. Contains 1 ATP-grasp domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Ligand | ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic processInferred from electronic annotation. Source: HAMAP |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 422 | 422 | Phosphoribosylamine--glycine ligase HAMAP MF_00138 | PRO_0000151464 | |||||
Regions | |||||||||
| Domain | 107 – 312 | 206 | ATP-grasp | ||||||
| Nucleotide binding | 137 – 193 | 57 | ATP By similarity | ||||||
Sites | |||||||||
| Metal binding | 282 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 284 | 1 | Magnesium or manganese By similarity | ||||||
Sequences
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References
| [1] | "Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G.Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv. |
| [2] | "Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M.J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh. |
Cross-references
Sequence databases | |
|---|---|
| BX842574 Genomic DNA. Translation: CAB02384.1. AE000516 Genomic DNA. Translation: AAK45038.1. | |
| PIR | G70707. |
| RefSeq | NP_215286.1. NP_335224.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GSO based on UniProtKB P15640. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 888873. 926099. |
| GenomeReviews | Gene locus MT0796 in contig AE000516_GR. Gene locus Rv0772 in contig AL123456_GR. |
| KEGG | mtc:MT0796. mtu:Rv0772. |
| TIGR | MT0796. |
Organism-specific databases | |
| TubercuList | Rv0772. |
Phylogenomic databases | |
| HOGENOM | P65893. |
| OMA | IEYNCRF. |
Enzyme and pathway databases | |
| BRENDA | 6.3.4.13. 809. |
Family and domain databases | |
| HAMAP | MF_00138. [Tree] |
| InterPro | IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view] |
| Gene3D | G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00877. purD. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUR2_MYCTU | ||||||||
| Accession | Primary (citable) accession number: P65893 Secondary accession number(s): P71827 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


