Reviewed, UniProtKB/Swiss-Prot P65879 (PURA_BRUSU)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	BR1683

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000095156
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	140	1	By similarity
	Active site	147	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65879-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: DF90E83E30690FF2	FASTA	429	46,561
	10 20 30 40 50 60 MANVVVVGSQ WGDEGKGKIV DWLSERADVI VRYQGGHNAG HTLVIDGVSY KLSLLPSGLV 70 80 90 100 110 120 RGKLSVIGNG VVVDPHHFVA EVEKLRGQGI DVTPDVLRVA ENAPLILSIH RELDAMREGS 130 140 150 160 170 180 NSGLKIGTTK RGIGPAYEDK VGRRAIRVID LTEPETLRPK VERLLAHHNS LRRGMGLEEI 190 200 210 220 230 240 AVETILTELT SVADQILPYI DQVWRVLDER RKAGARILFE GAQGALLDND HGTYPFVTSS 250 260 270 280 290 300 NTVAGQAAAG SGLGPTAIGY VLGITKAYTT RVGEGPFPTE LNDEIGEFLG TKGHEFGVVT 310 320 330 340 350 360 GRKRRCGWFD AVIVRQTVRT SGINGIALTK LDVLDGLEEI KICVAYELDG KRIDYLPSSM 370 380 390 400 410 420 GAQARVKPIY ETLPGWSETT AGARSWNDLP AQAVKYVRHI EELIGAPVAM LSTSPEREDT ILVTDPFHD

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN30583.1.
RefSeq	NP_698668.1.
3D structure databases
HSSP	HSSP built from PDB template 1ADE based on UniProtKB P12283.
ModBase	Search...
Genome annotation databases
GeneID	1167376.
GenomeReviews	Gene locus BR1683 in contig AE014291_GR.
KEGG	bms:BR1683.
TIGR	BR1683.
Phylogenomic databases
HOGENOM	P65879.
OMA	P65879. IPVCVAY.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_1683-MON.
BRENDA	6.3.4.4. 281610.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BRUSU

Accession

Primary (citable) accession number: P65879
Secondary accession number(s): Q8FZ20, Q8YIU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)