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Protein

6-carboxy-5,6,7,8-tetrahydropterin synthase

Gene

queD

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde.By similarity

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161ZincBy similarity
Active sitei27 – 271Proton acceptorBy similarity
Metal bindingi31 – 311ZincBy similarity
Metal bindingi33 – 331ZincBy similarity
Active sitei71 – 711Charge relay systemBy similarity
Active sitei110 – 1101Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
6-carboxy-5,6,7,8-tetrahydropterin synthase (EC:4.1.2.50)
Short name:
CPH4 synthase
Alternative name(s):
Queuosine biosynthesis protein QueD
Gene namesi
Name:queD
Synonyms:ygcM
Ordered Locus Names:SF2781, S2974
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1211216-carboxy-5,6,7,8-tetrahydropterin synthasePRO_0000057924Add
BLAST

Proteomic databases

PaxDbiP65872.

Interactioni

Protein-protein interaction databases

STRINGi198214.SF2781.

Structurei

3D structure databases

ProteinModelPortaliP65872.
SMRiP65872. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family. QueD subfamily.Curated

Phylogenomic databases

eggNOGiENOG4107ZIF. Bacteria.
COG0720. LUCA.
HOGENOMiHOG000225067.
KOiK01737.
OrthoDBiEOG6FFSCJ.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
TIGR03367. queuosine_QueD. 1 hit.

Sequencei

Sequence statusi: Complete.

P65872-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTTLFKDF TFEAAHRLPH VPEGHKCGRL HGHSFMVRLE ITGEVDPHTG
60 70 80 90 100
WIIDFAELKA AFKPTYERLD HHYLNDIPGL ENPTSEVLAK WIWDQVKPVV
110 120
PLLSAVMVKE TCTAGCIYRG E
Length:121
Mass (Da):13,773
Last modified:October 11, 2004 - v1
Checksum:iDEF4F2A2E72CFF0D
GO

Sequence cautioni

The sequence AAN44270.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAP18095.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44270.2. Different initiation.
AE014073 Genomic DNA. Translation: AAP18095.1. Different initiation.
RefSeqiNP_708563.2. NC_004337.2.

Genome annotation databases

EnsemblBacteriaiAAN44270; AAN44270; SF2781.
AAP18095; AAP18095; S2974.
GeneIDi1025773.
KEGGisfl:SF2781.
sfx:S2974.
PATRICi18707620. VBIShiFle31049_3265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44270.2. Different initiation.
AE014073 Genomic DNA. Translation: AAP18095.1. Different initiation.
RefSeqiNP_708563.2. NC_004337.2.

3D structure databases

ProteinModelPortaliP65872.
SMRiP65872. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF2781.

Proteomic databases

PaxDbiP65872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN44270; AAN44270; SF2781.
AAP18095; AAP18095; S2974.
GeneIDi1025773.
KEGGisfl:SF2781.
sfx:S2974.
PATRICi18707620. VBIShiFle31049_3265.

Phylogenomic databases

eggNOGiENOG4107ZIF. Bacteria.
COG0720. LUCA.
HOGENOMiHOG000225067.
KOiK01737.
OrthoDBiEOG6FFSCJ.

Enzyme and pathway databases

UniPathwayiUPA00391.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
TIGR03367. queuosine_QueD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiQUED_SHIFL
AccessioniPrimary (citable) accession number: P65872
Secondary accession number(s): Q46903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit (By similarity).By similarity

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.