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Protein

6-carboxy-5,6,7,8-tetrahydropterin synthase

Gene

queD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde. Can also convert 6-pyruvoyltetrahydropterin (PPH4) and sepiapterin to CPH4; these 2 compounds are probably intermediates in the reaction from H2NTP.1 Publication

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161ZincBy similarity
Active sitei27 – 271Proton acceptorBy similarity
Metal bindingi31 – 311ZincBy similarity
Metal bindingi33 – 331ZincBy similarity
Active sitei71 – 711Charge relay systemBy similarity
Active sitei110 – 1101Charge relay systemBy similarity

GO - Molecular functioni

  • 6-carboxy-5,6,7,8-tetrahydropterin synthase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7431-MONOMER.
ECOL316407:JW2735-MONOMER.
MetaCyc:G7431-MONOMER.
RETL1328306-WGS:GSTH-3787-MONOMER.
BRENDAi4.1.2.50. 2026.
UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
6-carboxy-5,6,7,8-tetrahydropterin synthase (EC:4.1.2.50)
Short name:
CPH4 synthase
Alternative name(s):
Queuosine biosynthesis protein QueD
Gene namesi
Name:queD
Synonyms:ygcM
Ordered Locus Names:b2765, JW2735
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13120. queD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1211216-carboxy-5,6,7,8-tetrahydropterin synthasePRO_0000057922Add
BLAST

Proteomic databases

PaxDbiP65870.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

IntActiP65870. 3 interactions.
MINTiMINT-1299113.
STRINGi511145.b2765.

Structurei

Secondary structure

1
121
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1614Combined sources
Helixi26 – 283Combined sources
Beta strandi29 – 4214Combined sources
Turni47 – 493Combined sources
Beta strandi50 – 534Combined sources
Helixi55 – 6915Combined sources
Helixi74 – 763Combined sources
Helixi85 – 9612Combined sources
Turni97 – 993Combined sources
Beta strandi103 – 1119Combined sources
Beta strandi114 – 1196Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QN0X-ray2.34A/B/C/D/E/F1-121[»]
3QN9X-ray2.93A/B1-121[»]
3QNAX-ray2.50A/B/C/D/E/F1-121[»]
4NTKX-ray1.60A/B/C/D/E/F1-121[»]
4NTMX-ray2.05A/B/C/D/E/F1-121[»]
4NTNX-ray1.99A/B/C/D/E/F1-121[»]
ProteinModelPortaliP65870.
SMRiP65870. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family. QueD subfamily.Curated

Phylogenomic databases

eggNOGiENOG4107ZIF. Bacteria.
COG0720. LUCA.
HOGENOMiHOG000225067.
InParanoidiP65870.
KOiK01737.
OMAiPNVPETH.
PhylomeDBiP65870.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
TIGR03367. queuosine_QueD. 1 hit.

Sequencei

Sequence statusi: Complete.

P65870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTTLFKDF TFEAAHRLPH VPEGHKCGRL HGHSFMVRLE ITGEVDPHTG
60 70 80 90 100
WIIDFAELKA AFKPTYERLD HHYLNDIPGL ENPTSEVLAK WIWDQVKPVV
110 120
PLLSAVMVKE TCTAGCIYRG E
Length:121
Mass (Da):13,773
Last modified:October 11, 2004 - v1
Checksum:iDEF4F2A2E72CFF0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29579 Genomic DNA. Translation: AAA69275.1.
U00096 Genomic DNA. Translation: AAC75807.1.
AP009048 Genomic DNA. Translation: BAE76842.1.
PIRiA65058.
RefSeqiNP_417245.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75807; AAC75807; b2765.
BAE76842; BAE76842; BAE76842.
GeneIDi945123.
KEGGiecj:JW2735.
eco:b2765.
PATRICi32120942. VBIEscCol129921_2863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29579 Genomic DNA. Translation: AAA69275.1.
U00096 Genomic DNA. Translation: AAC75807.1.
AP009048 Genomic DNA. Translation: BAE76842.1.
PIRiA65058.
RefSeqiNP_417245.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QN0X-ray2.34A/B/C/D/E/F1-121[»]
3QN9X-ray2.93A/B1-121[»]
3QNAX-ray2.50A/B/C/D/E/F1-121[»]
4NTKX-ray1.60A/B/C/D/E/F1-121[»]
4NTMX-ray2.05A/B/C/D/E/F1-121[»]
4NTNX-ray1.99A/B/C/D/E/F1-121[»]
ProteinModelPortaliP65870.
SMRiP65870. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP65870. 3 interactions.
MINTiMINT-1299113.
STRINGi511145.b2765.

Proteomic databases

PaxDbiP65870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75807; AAC75807; b2765.
BAE76842; BAE76842; BAE76842.
GeneIDi945123.
KEGGiecj:JW2735.
eco:b2765.
PATRICi32120942. VBIEscCol129921_2863.

Organism-specific databases

EchoBASEiEB2921.
EcoGeneiEG13120. queD.

Phylogenomic databases

eggNOGiENOG4107ZIF. Bacteria.
COG0720. LUCA.
HOGENOMiHOG000225067.
InParanoidiP65870.
KOiK01737.
OMAiPNVPETH.
PhylomeDBiP65870.

Enzyme and pathway databases

UniPathwayiUPA00391.
BioCyciEcoCyc:G7431-MONOMER.
ECOL316407:JW2735-MONOMER.
MetaCyc:G7431-MONOMER.
RETL1328306-WGS:GSTH-3787-MONOMER.
BRENDAi4.1.2.50. 2026.

Miscellaneous databases

PROiP65870.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
TIGR03367. queuosine_QueD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUED_ECOLI
AccessioniPrimary (citable) accession number: P65870
Secondary accession number(s): Q2MA64, Q46903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.