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Protein

6-carboxy-5,6,7,8-tetrahydropterin synthase

Gene

queD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde. Can also convert 6-pyruvoyltetrahydropterin (PPH4) and sepiapterin to CPH4; these 2 compounds are probably intermediates in the reaction from H2NTP.1 Publication

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit (By similarity).By similarity

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16ZincBy similarity1
Active sitei27Proton acceptorBy similarity1
Metal bindingi31ZincBy similarity1
Metal bindingi33ZincBy similarity1
Active sitei71Charge relay systemBy similarity1
Active sitei110Charge relay systemBy similarity1

GO - Molecular functioni

  • 6-carboxy-5,6,7,8-tetrahydropterin synthase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processQueuosine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7431-MONOMER
MetaCyc:G7431-MONOMER
BRENDAi4.1.2.50 2026
UniPathwayiUPA00391

Names & Taxonomyi

Protein namesi
Recommended name:
6-carboxy-5,6,7,8-tetrahydropterin synthase (EC:4.1.2.50)
Short name:
CPH4 synthase
Alternative name(s):
Queuosine biosynthesis protein QueD
Gene namesi
Name:queD
Synonyms:ygcM
Ordered Locus Names:b2765, JW2735
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13120 queD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579221 – 1216-carboxy-5,6,7,8-tetrahydropterin synthaseAdd BLAST121

Proteomic databases

PaxDbiP65870
PRIDEiP65870

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4262288, 21 interactors
IntActiP65870, 3 interactors
STRINGi316385.ECDH10B_2933

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 16Combined sources14
Helixi26 – 28Combined sources3
Beta strandi29 – 42Combined sources14
Turni47 – 49Combined sources3
Beta strandi50 – 53Combined sources4
Helixi55 – 69Combined sources15
Helixi74 – 76Combined sources3
Helixi85 – 96Combined sources12
Turni97 – 99Combined sources3
Beta strandi103 – 111Combined sources9
Beta strandi114 – 119Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QN0X-ray2.34A/B/C/D/E/F1-121[»]
3QN9X-ray2.93A/B1-121[»]
3QNAX-ray2.50A/B/C/D/E/F1-121[»]
4NTKX-ray1.60A/B/C/D/E/F1-121[»]
4NTMX-ray2.05A/B/C/D/E/F1-121[»]
4NTNX-ray1.99A/B/C/D/E/F1-121[»]
ProteinModelPortaliP65870
SMRiP65870
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family. QueD subfamily.Curated

Phylogenomic databases

eggNOGiENOG4107ZIF Bacteria
COG0720 LUCA
HOGENOMiHOG000225067
InParanoidiP65870
KOiK01737
OMAiKCEKLHG
PhylomeDBiP65870

Family and domain databases

Gene3Di3.30.479.10, 1 hit
InterProiView protein in InterPro
IPR007115 6-PTP_synth/QueD
IPR038418 6-PTP_synth/QueD_sf
PANTHERiPTHR12589 PTHR12589, 1 hit
PfamiView protein in Pfam
PF01242 PTPS, 1 hit
PIRSFiPIRSF006113 PTP_synth, 1 hit
TIGRFAMsiTIGR00039 6PTHBS, 1 hit
TIGR03367 queuosine_QueD, 1 hit

Sequencei

Sequence statusi: Complete.

P65870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTTLFKDF TFEAAHRLPH VPEGHKCGRL HGHSFMVRLE ITGEVDPHTG
60 70 80 90 100
WIIDFAELKA AFKPTYERLD HHYLNDIPGL ENPTSEVLAK WIWDQVKPVV
110 120
PLLSAVMVKE TCTAGCIYRG E
Length:121
Mass (Da):13,773
Last modified:October 11, 2004 - v1
Checksum:iDEF4F2A2E72CFF0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29579 Genomic DNA Translation: AAA69275.1
U00096 Genomic DNA Translation: AAC75807.1
AP009048 Genomic DNA Translation: BAE76842.1
PIRiA65058
RefSeqiNP_417245.1, NC_000913.3
WP_000987944.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75807; AAC75807; b2765
BAE76842; BAE76842; BAE76842
GeneIDi945123
KEGGiecj:JW2735
eco:b2765
PATRICifig|1411691.4.peg.3972

Similar proteinsi

Entry informationi

Entry nameiQUED_ECOLI
AccessioniPrimary (citable) accession number: P65870
Secondary accession number(s): Q2MA64, Q46903
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health