Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

6-carboxy-5,6,7,8-tetrahydropterin synthase

Gene

queD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde. Can also convert 6-pyruvoyltetrahydropterin (PPH4) and sepiapterin to CPH4; these 2 compounds are probably intermediates in the reaction from H2NTP.1 Publication

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16ZincBy similarity1
Active sitei27Proton acceptorBy similarity1
Metal bindingi31ZincBy similarity1
Metal bindingi33ZincBy similarity1
Active sitei71Charge relay systemBy similarity1
Active sitei110Charge relay systemBy similarity1

GO - Molecular functioni

  • 6-carboxy-5,6,7,8-tetrahydropterin synthase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7431-MONOMER.
ECOL316407:JW2735-MONOMER.
MetaCyc:G7431-MONOMER.
BRENDAi4.1.2.50. 2026.
UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
6-carboxy-5,6,7,8-tetrahydropterin synthase (EC:4.1.2.50)
Short name:
CPH4 synthase
Alternative name(s):
Queuosine biosynthesis protein QueD
Gene namesi
Name:queD
Synonyms:ygcM
Ordered Locus Names:b2765, JW2735
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13120. queD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579221 – 1216-carboxy-5,6,7,8-tetrahydropterin synthaseAdd BLAST121

Proteomic databases

PaxDbiP65870.
PRIDEiP65870.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

IntActiP65870. 3 interactors.
MINTiMINT-1299113.
STRINGi511145.b2765.

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 16Combined sources14
Helixi26 – 28Combined sources3
Beta strandi29 – 42Combined sources14
Turni47 – 49Combined sources3
Beta strandi50 – 53Combined sources4
Helixi55 – 69Combined sources15
Helixi74 – 76Combined sources3
Helixi85 – 96Combined sources12
Turni97 – 99Combined sources3
Beta strandi103 – 111Combined sources9
Beta strandi114 – 119Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QN0X-ray2.34A/B/C/D/E/F1-121[»]
3QN9X-ray2.93A/B1-121[»]
3QNAX-ray2.50A/B/C/D/E/F1-121[»]
4NTKX-ray1.60A/B/C/D/E/F1-121[»]
4NTMX-ray2.05A/B/C/D/E/F1-121[»]
4NTNX-ray1.99A/B/C/D/E/F1-121[»]
ProteinModelPortaliP65870.
SMRiP65870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family. QueD subfamily.Curated

Phylogenomic databases

eggNOGiENOG4107ZIF. Bacteria.
COG0720. LUCA.
HOGENOMiHOG000225067.
InParanoidiP65870.
KOiK01737.
OMAiPNVPETH.
PhylomeDBiP65870.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
TIGR03367. queuosine_QueD. 1 hit.

Sequencei

Sequence statusi: Complete.

P65870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTTLFKDF TFEAAHRLPH VPEGHKCGRL HGHSFMVRLE ITGEVDPHTG
60 70 80 90 100
WIIDFAELKA AFKPTYERLD HHYLNDIPGL ENPTSEVLAK WIWDQVKPVV
110 120
PLLSAVMVKE TCTAGCIYRG E
Length:121
Mass (Da):13,773
Last modified:October 11, 2004 - v1
Checksum:iDEF4F2A2E72CFF0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29579 Genomic DNA. Translation: AAA69275.1.
U00096 Genomic DNA. Translation: AAC75807.1.
AP009048 Genomic DNA. Translation: BAE76842.1.
PIRiA65058.
RefSeqiNP_417245.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75807; AAC75807; b2765.
BAE76842; BAE76842; BAE76842.
GeneIDi945123.
KEGGiecj:JW2735.
eco:b2765.
PATRICi32120942. VBIEscCol129921_2863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29579 Genomic DNA. Translation: AAA69275.1.
U00096 Genomic DNA. Translation: AAC75807.1.
AP009048 Genomic DNA. Translation: BAE76842.1.
PIRiA65058.
RefSeqiNP_417245.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QN0X-ray2.34A/B/C/D/E/F1-121[»]
3QN9X-ray2.93A/B1-121[»]
3QNAX-ray2.50A/B/C/D/E/F1-121[»]
4NTKX-ray1.60A/B/C/D/E/F1-121[»]
4NTMX-ray2.05A/B/C/D/E/F1-121[»]
4NTNX-ray1.99A/B/C/D/E/F1-121[»]
ProteinModelPortaliP65870.
SMRiP65870.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP65870. 3 interactors.
MINTiMINT-1299113.
STRINGi511145.b2765.

Proteomic databases

PaxDbiP65870.
PRIDEiP65870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75807; AAC75807; b2765.
BAE76842; BAE76842; BAE76842.
GeneIDi945123.
KEGGiecj:JW2735.
eco:b2765.
PATRICi32120942. VBIEscCol129921_2863.

Organism-specific databases

EchoBASEiEB2921.
EcoGeneiEG13120. queD.

Phylogenomic databases

eggNOGiENOG4107ZIF. Bacteria.
COG0720. LUCA.
HOGENOMiHOG000225067.
InParanoidiP65870.
KOiK01737.
OMAiPNVPETH.
PhylomeDBiP65870.

Enzyme and pathway databases

UniPathwayiUPA00391.
BioCyciEcoCyc:G7431-MONOMER.
ECOL316407:JW2735-MONOMER.
MetaCyc:G7431-MONOMER.
BRENDAi4.1.2.50. 2026.

Miscellaneous databases

PROiP65870.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
PIRSFiPIRSF006113. PTP_synth. 1 hit.
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
TIGR03367. queuosine_QueD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUED_ECOLI
AccessioniPrimary (citable) accession number: P65870
Secondary accession number(s): Q2MA64, Q46903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.