P65861 (CRTB_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phytoene synthase
EC=2.5.1.32

Gene names

Name:	crtB
Synonyms:	phyA
Ordered Locus Names:	Mb3430c

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene.
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Probable phytoene synthase		PRO_0000067432

Sequences

	Sequence		Length	Mass (Da)
	P65861 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C214605A7B93AFFD	FASTA	302	33,142
	10 20 30 40 50 60 MTEIEQAYRI TESITRTAAR NFYYGIRLLP REKRAALSAV YALGRRIDDV ADGELAPETK 70 80 90 100 110 120 ITELDAIRKS LDNIDDSSDP VLVALADAAR RFPVPIAMFA ELIDGARMEI DWTGCRDFDE 130 140 150 160 170 180 LIVYCRRGAG TIGKLCLSIF GPVSTATSRY AEQLGIALQQ TNILRDVRED FLNGRIYLPR 190 200 210 220 230 240 DELDRLGVRL RLDDTGALDD PDGRLAALLR FSADRAADWY SLGLRLIPHL DRRSAACCAA 250 260 270 280 290 300 MSGIYRRQLA LIRASPAVVY DRRISLSGLK KAQVAAAALA SSVTCGPAHG PLPADLGSHP SH

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248346 Genomic DNA. Translation: CAD95618.1.
RefSeq	NP_857071.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P65861.
SMR	P65861. Positions 1-260.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb3430c.
Proteomic databases
PRIDE	P65861.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD95618; CAD95618; Mb3430c.
GeneID	1090989.
KEGG	mbo:Mb3430c.
PATRIC	18009274. VBIMycBov88188_3765.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1562.
HOGENOM	HOG000220849.
KO	K02291.
OMA	EWVEAFL.
ProtClustDB	CLSK872176.
Enzyme and pathway databases
UniPathway	UPA00799; UER00773.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR017828. Squalene/phytoene_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03465. HpnD. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTB_MYCBO

Accession

Primary (citable) accession number: P65861
Secondary accession number(s): Q50728

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program