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Protein

Phytoene synthase

Gene

crtB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield phytoene (Probable).Curated

Cofactori

Protein has several cofactor binding sites:
  • ATPBy similarityNote: ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.By similarity
  • Mn2+By similarity, Mg2+By similarity

Pathway: phytoene biosynthesis

This protein is involved in the pathway phytoene biosynthesis, which is part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene synthase (EC:2.5.1.-)
Short name:
PSase
Gene namesi
Name:crtB
Synonyms:phyA
Ordered Locus Names:Mb3430c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Phytoene synthasePRO_0000067432Add
BLAST

Proteomic databases

PRIDEiP65861.

Structurei

3D structure databases

ProteinModelPortaliP65861.
SMRiP65861. Positions 1-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220849.
KOiK02291.
OMAiAYSYCEA.
OrthoDBiEOG63FW00.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR017828. Squalene/phytoene_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03465. HpnD. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65861-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEIEQAYRI TESITRTAAR NFYYGIRLLP REKRAALSAV YALGRRIDDV
60 70 80 90 100
ADGELAPETK ITELDAIRKS LDNIDDSSDP VLVALADAAR RFPVPIAMFA
110 120 130 140 150
ELIDGARMEI DWTGCRDFDE LIVYCRRGAG TIGKLCLSIF GPVSTATSRY
160 170 180 190 200
AEQLGIALQQ TNILRDVRED FLNGRIYLPR DELDRLGVRL RLDDTGALDD
210 220 230 240 250
PDGRLAALLR FSADRAADWY SLGLRLIPHL DRRSAACCAA MSGIYRRQLA
260 270 280 290 300
LIRASPAVVY DRRISLSGLK KAQVAAAALA SSVTCGPAHG PLPADLGSHP

SH
Length:302
Mass (Da):33,142
Last modified:October 11, 2004 - v1
Checksum:iC214605A7B93AFFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44702.1.
RefSeqiNP_857071.1. NC_002945.3.
WP_003417954.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO44702; CDO44702; Mb3430c.
GeneIDi1090989.
KEGGimbo:Mb3430c.
PATRICi18009274. VBIMycBov88188_3765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44702.1.
RefSeqiNP_857071.1. NC_002945.3.
WP_003417954.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP65861.
SMRiP65861. Positions 1-226.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP65861.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO44702; CDO44702; Mb3430c.
GeneIDi1090989.
KEGGimbo:Mb3430c.
PATRICi18009274. VBIMycBov88188_3765.

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220849.
KOiK02291.
OMAiAYSYCEA.
OrthoDBiEOG63FW00.

Enzyme and pathway databases

UniPathwayiUPA00799.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR017828. Squalene/phytoene_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03465. HpnD. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiCRTB_MYCBO
AccessioniPrimary (citable) accession number: P65861
Secondary accession number(s): Q50728, X2BN51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 24, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.