P65841 (RSUA_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal small subunit pseudouridine synthase A
EC=5.4.99.19

Alternative name(s):
16S pseudouridylate 516 synthase
16S rRNA pseudouridine(516) synthase
rRNA pseudouridylate synthase A
rRNA-uridine isomerase A

Gene names

Name:	rsuA
Ordered Locus Names:	STY2459, t0632

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA By similarity.
Catalytic activity	16S rRNA uridine(516) = 16S rRNA pseudouridine(516).
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the pseudouridine synthase RsuA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Ribosomal small subunit pseudouridine synthase A		PRO_0000099972
Regions
	Domain	1 – 68	68	S4 RNA-binding
Sites
	Active site	102	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65841 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 29EF9A7C5BD1DEF5	FASTA	231	25,760
	10 20 30 40 50 60 MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GDIIKNAAFK LLPEHAVAYD GNPLAQQHGP 70 80 90 100 110 120 RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAYKLHAAGR LDIDTTGLVL MTDDGQWSHR 130 140 150 160 170 180 ITSPRHHCEK TYLVTLESPV ADDTAAQFAK GVQLHNEKDL TKPATLEVIT PVQVRLTISE 190 200 210 220 230 GRYHQVKRMF AAVGNRVVEL HRERIGAITL DENLAPGEYR PLTEEEIASV G

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627273 Genomic DNA. Translation: CAD02605.1. AE014613 Genomic DNA. Translation: AAO68333.1.
RefSeq	NP_804484.1. NC_004631.1.
3D structure databases
ProteinModelPortal	P65841.
SMR	P65841. Positions 1-230.
ModBase	Search...
Protein-protein interaction databases
STRING	220341.STY2459.
Proteomic databases
PRIDE	P65841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO68333; AAO68333; t0632. CAD02605; CAD02605; CAD02605.
GeneID	1069758.
KEGG	stt:t0632.
PATRIC	18542875. VBISalEnt120419_2487.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1187.
HOGENOM	HOG000044954.
KO	K06183.
OMA	IGPRFIM.
ProtClustDB	PRK10839.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR000748. PsdUridine_synth_RsuA/RluB/E/F. IPR018496. PsdUridine_synth_RsuA/RluB_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00093. TIGR00093. 1 hit.
PROSITE	PS01149. PSI_RSU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSUA_SALTI

Accession

Primary (citable) accession number: P65841
Secondary accession number(s): Q8XGP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program