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P65831

- PUR1_STAAM

UniProt

P65831 - PUR1_STAAM

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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111NucleophileUniRule annotation
Metal bindingi294 – 2941MagnesiumUniRule annotation
Metal bindingi356 – 3561MagnesiumUniRule annotation
Metal bindingi357 – 3571MagnesiumUniRule annotation

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1089-MONOMER.
UniPathwayiUPA00074; UER00124.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:SAV1070
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1010By similarityPRO_0000029263
Chaini11 – 494484AmidophosphoribosyltransferasePRO_0000029264Add
BLAST

Proteomic databases

PRIDEiP65831.

2D gel databases

World-2DPAGE0002:P65831.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1070.

Structurei

3D structure databases

ProteinModelPortaliP65831.
SMRiP65831. Positions 11-461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 231221Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiSGIPYEM.
OrthoDBiEOG6KT2Q1.
PhylomeDBiP65831.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P65831-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSDQNE
60 70 80 90 100
LKGERGLGLL TEAINDDQME RLKGYQHAIG HVRYATSGNK GIENIQPFLY
110 120 130 140 150
HFYDMSVGIC HNGNLINAKS LRQNLEKQGA IFHSSSDTEV IMHLIRRSKA
160 170 180 190 200
PTFEEALKES LRKVKGGFTF AILTKDALYG AVDPNAIRPL VVGKMKDGTY
210 220 230 240 250
ILASETCAID VLGAEFVQDI HAGEYVVIND KGITVKSYTH HTTTAISAME
260 270 280 290 300
YIYFARPDST IAGKNVHAVR KASGKKLAQE SPVNADMVIG VPNSSLSAAS
310 320 330 340 350
GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK
360 370 380 390 400
NIILVDDSIV RGTTIRRIVK MLKDSGANKV HVRIASPEFM FPSFYGIDVS
410 420 430 440 450
TTAELISASK SPEEIKDYIG ADSLAYLSVD GLIESIGLDY DAPYSGLCVE
460 470 480 490
SFTGDYPAGL YDYEANYKAH LSHRQKQYIS KNKHFFDSEG NLNV
Length:494
Mass (Da):54,383
Last modified:October 11, 2004 - v1
Checksum:iCCF5D947C381646D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57232.1.
RefSeqiNP_371594.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57232; BAB57232; SAV1070.
GeneIDi1121047.
KEGGisav:SAV1070.
PATRICi19562839. VBIStaAur52173_1098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57232.1 .
RefSeqi NP_371594.1. NC_002758.2.

3D structure databases

ProteinModelPortali P65831.
SMRi P65831. Positions 11-461.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 158878.SAV1070.

2D gel databases

World-2DPAGE 0002:P65831.

Proteomic databases

PRIDEi P65831.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB57232 ; BAB57232 ; SAV1070 .
GeneIDi 1121047.
KEGGi sav:SAV1070.
PATRICi 19562839. VBIStaAur52173_1098.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033688.
KOi K00764.
OMAi SGIPYEM.
OrthoDBi EOG6KT2Q1.
PhylomeDBi P65831.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci SAUR158878:GJJ5-1089-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPi MF_01931. PurF.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiPUR1_STAAM
AccessioniPrimary (citable) accession number: P65831
Secondary accession number(s): Q99V27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3