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P65830 (PUR1_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:Mb0831
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 3434 By similarity
PRO_0000029259
Chain35 – 527493Amidophosphoribosyltransferase
PRO_0000029260

Regions

Domain35 – 261227Glutamine amidotransferase type-2

Sites

Active site351For GATase activity By similarity
Metal binding2761Iron-sulfur (4Fe-4S) By similarity
Metal binding3231Magnesium By similarity
Metal binding3851Magnesium By similarity
Metal binding3861Magnesium By similarity
Metal binding4221Iron-sulfur (4Fe-4S) By similarity
Metal binding4781Iron-sulfur (4Fe-4S) By similarity
Metal binding4811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P65830 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: A2FE9D1DD08FA572

FASTA52756,162
        10         20         30         40         50         60 
MAVDSDYVTD RAAGSRQTVT GQQPEQDLNS PREECGVFGV WAPGEDVAKL TYYGLYALQH 

        70         80         90        100        110        120 
RGQEAAGIAV ADGSQVLVFK DLGLVSQVFD EQTLAAMQGH VAIGHCRYST TGDTTWENAQ 

       130        140        150        160        170        180 
PVFRNTAAGT GVALGHNGNL VNAAALAARA RDAGLIATRC PAPATTDSDI LGALLAHGAA 

       190        200        210        220        230        240 
DSTLEQAALD LLPTVRGAFC LTFMDENTLY ACRDPYGVRP LSLGRLDRGW VVASETAALD 

       250        260        270        280        290        300 
IVGASFVRDI EPGELLAIDA DGVRSTRFAN PTPKGCVFEY VYLARPDSTI AGRSVHAARV 

       310        320        330        340        350        360 
EIGRRLAREC PVEADLVIGV PESGTPAAVG YAQESGVPYG QGLMKNAYVG RTFIQPSQTI 

       370        380        390        400        410        420 
RQLGIRLKLN PLKEVIRGKR LIVVDDSIVR GNTQRALVRM LREAGAVELH VRIASPPVKW 

       430        440        450        460        470        480 
PCFYGIDFPS PAELIANAVE NEDEMLEAVR HAIGADTLGY ISLRGMVAAS EQPTSRLCTA 

       490        500        510        520 
CFDGKYPIEL PRETALGKNV IEHMLANAAR GAALGELAAD DEVPVGR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248336 Genomic DNA. Translation: CAD93693.1.
RefSeqNP_854489.1. NC_002945.3.

3D structure databases

ProteinModelPortalP65830.
SMRP65830. Positions 35-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb0831.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD93693; CAD93693; Mb0831.
GeneID1092560.
KEGGmbo:Mb0831.
PATRIC18003482. VBIMycBov88188_0903.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
KOK00764.
OMACDACFTG.
OrthoDBEOG6KT2Q1.
ProtClustDBPRK07847.

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_MYCBO
AccessionPrimary (citable) accession number: P65830
Secondary accession number(s): O06626
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 13, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways