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P65830

- PUR1_MYCBO

UniProt

P65830 - PUR1_MYCBO

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei35 – 351For GATase activityBy similarity
    Metal bindingi276 – 2761Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi323 – 3231MagnesiumBy similarity
    Metal bindingi385 – 3851MagnesiumBy similarity
    Metal bindingi386 – 3861MagnesiumBy similarity
    Metal bindingi422 – 4221Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi478 – 4781Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi481 – 4811Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:Mb0831
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 3434By similarityPRO_0000029259Add
    BLAST
    Chaini35 – 527493AmidophosphoribosyltransferasePRO_0000029260Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb0831.

    Structurei

    3D structure databases

    ProteinModelPortaliP65830.
    SMRiP65830. Positions 35-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 261227Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    KOiK00764.
    OMAiQYPIELP.
    OrthoDBiEOG6KT2Q1.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P65830-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVDSDYVTD RAAGSRQTVT GQQPEQDLNS PREECGVFGV WAPGEDVAKL    50
    TYYGLYALQH RGQEAAGIAV ADGSQVLVFK DLGLVSQVFD EQTLAAMQGH 100
    VAIGHCRYST TGDTTWENAQ PVFRNTAAGT GVALGHNGNL VNAAALAARA 150
    RDAGLIATRC PAPATTDSDI LGALLAHGAA DSTLEQAALD LLPTVRGAFC 200
    LTFMDENTLY ACRDPYGVRP LSLGRLDRGW VVASETAALD IVGASFVRDI 250
    EPGELLAIDA DGVRSTRFAN PTPKGCVFEY VYLARPDSTI AGRSVHAARV 300
    EIGRRLAREC PVEADLVIGV PESGTPAAVG YAQESGVPYG QGLMKNAYVG 350
    RTFIQPSQTI RQLGIRLKLN PLKEVIRGKR LIVVDDSIVR GNTQRALVRM 400
    LREAGAVELH VRIASPPVKW PCFYGIDFPS PAELIANAVE NEDEMLEAVR 450
    HAIGADTLGY ISLRGMVAAS EQPTSRLCTA CFDGKYPIEL PRETALGKNV 500
    IEHMLANAAR GAALGELAAD DEVPVGR 527
    Length:527
    Mass (Da):56,162
    Last modified:October 11, 2004 - v1
    Checksum:iA2FE9D1DD08FA572
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO42076.1.
    RefSeqiNP_854489.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD93693; CAD93693; Mb0831.
    GeneIDi1092560.
    KEGGimbo:Mb0831.
    PATRICi18003482. VBIMycBov88188_0903.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO42076.1 .
    RefSeqi NP_854489.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali P65830.
    SMRi P65830. Positions 35-496.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb0831.

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD93693 ; CAD93693 ; Mb0831 .
    GeneIDi 1092560.
    KEGGi mbo:Mb0831.
    PATRICi 18003482. VBIMycBov88188_0903.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    KOi K00764.
    OMAi QYPIELP.
    OrthoDBi EOG6KT2Q1.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiPUR1_MYCBO
    AccessioniPrimary (citable) accession number: P65830
    Secondary accession number(s): O06626, X2BG87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3