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P65830

- PUR1_MYCBO

UniProt

P65830 - PUR1_MYCBO

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Protein
Amidophosphoribosyltransferase
Gene
purF, Mb0831
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 4Fe-4S cluster per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei35 – 351For GATase activity By similarity
Metal bindingi276 – 2761Iron-sulfur (4Fe-4S) By similarity
Metal bindingi323 – 3231Magnesium By similarity
Metal bindingi385 – 3851Magnesium By similarity
Metal bindingi386 – 3861Magnesium By similarity
Metal bindingi422 – 4221Iron-sulfur (4Fe-4S) By similarity
Metal bindingi478 – 4781Iron-sulfur (4Fe-4S) By similarity
Metal bindingi481 – 4811Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
Ordered Locus Names:Mb0831
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 3434 By similarity
PRO_0000029259Add
BLAST
Chaini35 – 527493Amidophosphoribosyltransferase
PRO_0000029260Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb0831.

Structurei

3D structure databases

ProteinModelPortaliP65830.
SMRiP65830. Positions 35-496.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 261227Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiQYPIELP.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P65830-1 [UniParc]FASTAAdd to Basket

« Hide

MAVDSDYVTD RAAGSRQTVT GQQPEQDLNS PREECGVFGV WAPGEDVAKL    50
TYYGLYALQH RGQEAAGIAV ADGSQVLVFK DLGLVSQVFD EQTLAAMQGH 100
VAIGHCRYST TGDTTWENAQ PVFRNTAAGT GVALGHNGNL VNAAALAARA 150
RDAGLIATRC PAPATTDSDI LGALLAHGAA DSTLEQAALD LLPTVRGAFC 200
LTFMDENTLY ACRDPYGVRP LSLGRLDRGW VVASETAALD IVGASFVRDI 250
EPGELLAIDA DGVRSTRFAN PTPKGCVFEY VYLARPDSTI AGRSVHAARV 300
EIGRRLAREC PVEADLVIGV PESGTPAAVG YAQESGVPYG QGLMKNAYVG 350
RTFIQPSQTI RQLGIRLKLN PLKEVIRGKR LIVVDDSIVR GNTQRALVRM 400
LREAGAVELH VRIASPPVKW PCFYGIDFPS PAELIANAVE NEDEMLEAVR 450
HAIGADTLGY ISLRGMVAAS EQPTSRLCTA CFDGKYPIEL PRETALGKNV 500
IEHMLANAAR GAALGELAAD DEVPVGR 527
Length:527
Mass (Da):56,162
Last modified:October 11, 2004 - v1
Checksum:iA2FE9D1DD08FA572
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO42076.1.
RefSeqiNP_854489.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD93693; CAD93693; Mb0831.
GeneIDi1092560.
KEGGimbo:Mb0831.
PATRICi18003482. VBIMycBov88188_0903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO42076.1 .
RefSeqi NP_854489.1. NC_002945.3.

3D structure databases

ProteinModelPortali P65830.
SMRi P65830. Positions 35-496.
ModBasei Search...

Protein-protein interaction databases

STRINGi 233413.Mb0831.

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD93693 ; CAD93693 ; Mb0831 .
GeneIDi 1092560.
KEGGi mbo:Mb0831.
PATRICi 18003482. VBIMycBov88188_0903.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033688.
KOi K00764.
OMAi QYPIELP.
OrthoDBi EOG6KT2Q1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPUR1_MYCBO
AccessioniPrimary (citable) accession number: P65830
Secondary accession number(s): O06626, X2BG87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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