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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei35 – 351NucleophileUniRule annotation
Metal bindingi276 – 2761Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi323 – 3231MagnesiumUniRule annotation
Metal bindingi385 – 3851MagnesiumUniRule annotation
Metal bindingi386 – 3861MagnesiumUniRule annotation
Metal bindingi422 – 4221Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi478 – 4781Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi481 – 4811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:Mb0831
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 3434By similarityPRO_0000029259Add
BLAST
Chaini35 – 527493AmidophosphoribosyltransferasePRO_0000029260Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb0831.

Structurei

3D structure databases

ProteinModelPortaliP65830.
SMRiP65830. Positions 35-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 261227Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P65830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVDSDYVTD RAAGSRQTVT GQQPEQDLNS PREECGVFGV WAPGEDVAKL
60 70 80 90 100
TYYGLYALQH RGQEAAGIAV ADGSQVLVFK DLGLVSQVFD EQTLAAMQGH
110 120 130 140 150
VAIGHCRYST TGDTTWENAQ PVFRNTAAGT GVALGHNGNL VNAAALAARA
160 170 180 190 200
RDAGLIATRC PAPATTDSDI LGALLAHGAA DSTLEQAALD LLPTVRGAFC
210 220 230 240 250
LTFMDENTLY ACRDPYGVRP LSLGRLDRGW VVASETAALD IVGASFVRDI
260 270 280 290 300
EPGELLAIDA DGVRSTRFAN PTPKGCVFEY VYLARPDSTI AGRSVHAARV
310 320 330 340 350
EIGRRLAREC PVEADLVIGV PESGTPAAVG YAQESGVPYG QGLMKNAYVG
360 370 380 390 400
RTFIQPSQTI RQLGIRLKLN PLKEVIRGKR LIVVDDSIVR GNTQRALVRM
410 420 430 440 450
LREAGAVELH VRIASPPVKW PCFYGIDFPS PAELIANAVE NEDEMLEAVR
460 470 480 490 500
HAIGADTLGY ISLRGMVAAS EQPTSRLCTA CFDGKYPIEL PRETALGKNV
510 520
IEHMLANAAR GAALGELAAD DEVPVGR
Length:527
Mass (Da):56,162
Last modified:October 10, 2004 - v1
Checksum:iA2FE9D1DD08FA572
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO42076.1.
RefSeqiNP_854489.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO42076; CDO42076; Mb0831.
GeneIDi1092560.
KEGGimbo:Mb0831.
PATRICi18003482. VBIMycBov88188_0903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO42076.1.
RefSeqiNP_854489.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP65830.
SMRiP65830. Positions 35-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233413.Mb0831.

Protein family/group databases

MEROPSiC44.A03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO42076; CDO42076; Mb0831.
GeneIDi1092560.
KEGGimbo:Mb0831.
PATRICi18003482. VBIMycBov88188_0903.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPUR1_MYCBO
AccessioniPrimary (citable) accession number: P65830
Secondary accession number(s): O06626, X2BG87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2004
Last sequence update: October 10, 2004
Last modified: March 31, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.