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P65827

- HPRT_STAAM

UniProt

P65827 - HPRT_STAAM

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

hpt

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei102 – 1021Proton acceptorBy similarity
    Binding sitei130 – 1301IMPBy similarity
    Binding sitei152 – 1521IMP; via carbonyl oxygenBy similarity
    Metal bindingi158 – 1581MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi98 – 10710IMPBy similarity
    Nucleotide bindingi157 – 1582IMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. IMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAUR158878:GJJ5-514-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:hpt
    Ordered Locus Names:SAV0510
    OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
    Taxonomic identifieri158878 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002481: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139613Add
    BLAST

    Proteomic databases

    PRIDEiP65827.

    2D gel databases

    World-2DPAGE0002:P65827.

    Interactioni

    Protein-protein interaction databases

    STRINGi158878.SAV0510.

    Structurei

    3D structure databases

    ProteinModelPortaliP65827.
    SMRiP65827. Positions 4-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000236520.
    KOiK00760.
    OMAiVDFMTVS.
    OrthoDBiEOG693GNP.
    PhylomeDBiP65827.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P65827-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHNDLKEVLL TEEDIQNICK ELGAQLTKDY QGKPLVCVGI LKGSAMFMSD    50
    LIKRIDTHLS IDFMDVSSYH GGTESTGEVQ IIKDLGSSIE NKDVLIIEDI 100
    LETGTTLKSI TELLQSRKVN SLEIVTLLDK PNRRKADIEA KYVGKKIPDE 150
    FVVGYGLDYR ELYRNLPYIG TLKPEVYSN 179
    Length:179
    Mass (Da):20,154
    Last modified:October 11, 2004 - v1
    Checksum:iE7D35987B435EFB0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB56672.1.
    RefSeqiNP_371034.1. NC_002758.2.

    Genome annotation databases

    EnsemblBacteriaiBAB56672; BAB56672; SAV0510.
    GeneIDi1120470.
    KEGGisav:SAV0510.
    PATRICi19561642. VBIStaAur52173_0514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB56672.1 .
    RefSeqi NP_371034.1. NC_002758.2.

    3D structure databases

    ProteinModelPortali P65827.
    SMRi P65827. Positions 4-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158878.SAV0510.

    2D gel databases

    World-2DPAGE 0002:P65827.

    Proteomic databases

    PRIDEi P65827.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB56672 ; BAB56672 ; SAV0510 .
    GeneIDi 1120470.
    KEGGi sav:SAV0510.
    PATRICi 19561642. VBIStaAur52173_0514.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000236520.
    KOi K00760.
    OMAi VDFMTVS.
    OrthoDBi EOG693GNP.
    PhylomeDBi P65827.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci SAUR158878:GJJ5-514-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mu50 / ATCC 700699.

    Entry informationi

    Entry nameiHPRT_STAAM
    AccessioniPrimary (citable) accession number: P65827
    Secondary accession number(s): Q99W93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3