P65825 (SSPP_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Staphopain A
EC=3.4.22.48

Alternative name(s):
Staphylococcal cysteine proteinase A
Staphylopain A

Gene names

Name:	sspP
Synonyms:	scpA
Ordered Locus Names:	SAV1909

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cysteine protease able to degrade elastin By similarity.
Catalytic activity	Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate.
Enzyme regulation	Prematurely activated/folded staphopain A is inhibited by staphostatin A (scpB), which is probably required to protect staphylococcal cytoplasmic proteins from degradation by scpA By similarity.
Subunit structure	In the cytoplasm, prematurely activated/folded scpA forms a stable non-covalent complex with scpB By similarity.
Subcellular location	Secreted By similarity.
Post-translational modification	Cleavage leads to the activation of scpA probably by an auto-catalytic manner By similarity.
Miscellaneous	The catalytic maturation of scpA appears to reside outside the cascade of activation started by the metalloprotease aureolysin (aur) By similarity.
Sequence similarities	Belongs to the peptidase C47 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 214	189	By similarity		PRO_0000026549
	Chain	215 – 388	174	Staphopain A		PRO_0000026550
Sites
	Active site	238	1	By similarity
	Active site	334	1	By similarity
	Active site	355	1	By similarity
	Site	214 – 215	2	Cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65825 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 08540023411772CF	FASTA	388	44,204
	10 20 30 40 50 60 MKRNFPKLIA LSLIFSLSVT PIANAESNSN IKAKDKKHVQ VNVEDKSVPT DVRNLAQKDY 70 80 90 100 110 120 LSYVTSLDKI YNKEKASYTL GEPFKIYKFN KKSDGNYYFP VLNTEGNIDY IVTISPKITK 130 140 150 160 170 180 YSSSSSKYTI NVSPFLSKVL NQYKDQQITI LTNSKGYYVV TQNHKAKLVL KTPRLEDKKL 190 200 210 220 230 240 KKTESIPTGN NVTQLKQKAS VTMPTSQFKS NNYTYNEQYI NKLENFKIRE TQGNNGWCAG 250 260 270 280 290 300 YTMSALLNAT YNTNKYHAEA VMRFLHPNLQ GQRFQFTGLT PREMIYFGQT QGRSPQLLNR 310 320 330 340 350 360 MTTYNEVDNL TKNNKGIAVL GSRVESRNGM HAGHAMAVVG NAKLDNGQEV IIIWNPWDNG 370 380 FMTQDAKNNV IPVSNGDHYR WYSSIYGY

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB58071.1.
RefSeq	NP_372433.1. NC_002758.2.
3D structure databases
ProteinModelPortal	P65825.
SMR	P65825. Positions 216-388.
ModBase	Search...
Protein-protein interaction databases
STRING	P65825.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000005963; EBSTAP00000005781; EBSTAG00000005962.
GeneID	1121922.
GenomeReviews	Gene locus SAV1909 in contig BA000017_GR.
KEGG	sav:SAV1909.
PATRIC	19564672. VBIStaAur52173_1976.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG43315.
GeneTree	EBGT00050000023841.
HOGENOM	HBG217891.
OMA	YTINVSS.
ProtClustDB	CLSK885098.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV1909-MONOMER.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR008750. Peptidase_C47. [Graphical view]
KO	K08258.
Pfam	PF05543. Peptidase_C47. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. False negative. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSPP_STAAM

Accession

Primary (citable) accession number: P65825
Secondary accession number(s): Q99SX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program