P65815 (HTPX_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protease HtpX homolog
EC=3.4.24.-

Gene names

Name:	htpX
Ordered Locus Names:	Rv0563, MT0589
ORF Names:	MTCY25D10.42, MTV039.01

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00188.
Biotechnological use	Mice immunized with recombinant bacteria carrying a DNA vaccine encoding HtpX were significantly protected from challenge with M.tuberculosis, indicating this might be a good vaccine candidate. Ref.3
Sequence similarities	Belongs to the peptidase M48B family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from direct assay PubMed 17443846. Source: MTBBASE integral to plasma membrane Inferred from direct assay PubMed 15952732. Source: MTBBASE
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Protease HtpX homolog HAMAP-Rule MF_00188		PRO_0000138875
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	145 – 165	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
Sites
	Active site	136	1	By similarity
	Metal binding	135	1	Zinc; catalytic By similarity
	Metal binding	139	1	Zinc; catalytic By similarity
	Metal binding	206	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65815 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 9D1ED210A449465D	FASTA	286	30,682
	10 20 30 40 50 60 MTWHPHANRL KTFLLLVGMS ALIVAVGALF GRTALMLAAL FAVGMNVYVY FNSDKLALRA 70 80 90 100 110 120 MHAQPVSELQ APAMYRIVRE LATSAHQPMP RLYISDTAAP NAFATGRNPR NAAVCCTTGI 130 140 150 160 170 180 LRILNERELR AVLGHELSHV YNRDILISCV AGALAAVITA LANMAMWAGM FGGNRDNANP 190 200 210 220 230 240 FALLLVALLG PIAATVIRMA VSRSREYQAD ESGAVLTGDP LALASALRKI SGGVQAAPLP 250 260 270 280 PEPQLASQAH LMIANPFRAG ERIGSLFSTH PPIEDRIRRL EAMARG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Intranasal delivery of DNA encoding antigens of Mycobacterium tuberculosis by non-pathogenic invasive Escherichia coli." Brun P., Zumbo A., Castagliuolo I., Delogu G., Manfrin F., Sali M., Fadda G., Grillot-Courvalin C., Palu G., Manganelli R. Vaccine 26:1934-1941(2008) [PubMed] [Europe PMC] [Abstract] Cited for: BIOTECHNOLOGY. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842573 Genomic DNA. Translation: CAB08997.1. AE000516 Genomic DNA. Translation: AAK44812.1. AL123456 Genomic DNA. Translation: CCP43301.1.
PIR	F70549.
RefSeq	NP_215077.1. NC_000962.3. NP_334998.1. NC_002755.2. YP_006513896.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P65815.
SMR	P65815. Positions 56-146.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv0563.
Protein family/group databases
MEROPS	M48.004.
Proteomic databases
PRIDE	P65815.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK44812; AAK44812; MT0589.
GeneID	13318437. 887649. 924973.
KEGG	mtc:MT0589. mtu:Rv0563. mtv:RVBD_0563.
PATRIC	18122994. VBIMycTub22151_0631.
Organism-specific databases
TubercuList	Rv0563.
Phylogenomic databases
eggNOG	COG0501.
HOGENOM	HOG000227301.
KO	K03799.
OMA	RRGAEIC.
ProtClustDB	PRK03072.
Family and domain databases
HAMAP	MF_00188. Peptidase_M48.
InterPro	IPR022919. Pept_M48_protease_HtpX. IPR001915. Peptidase_M48. [Graphical view]
Pfam	PF01435. Peptidase_M48. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HTPX_MYCTU

Accession

Primary (citable) accession number: P65815
Secondary accession number(s): L0T459, O06429

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program