P65805 (PEPT_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidase T
EC=3.4.11.4

Alternative name(s):
Aminotripeptidase
Short name=Tripeptidase
Tripeptide aminopeptidase

Gene names

Name:	pepT
Ordered Locus Names:	SAV0743

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves the N-terminal amino acid of tripeptides By similarity. HAMAP-Rule MF_00550
Catalytic activity	Release of the N-terminal residue from a tripeptide. HAMAP-Rule MF_00550
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP-Rule MF_00550
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00550.
Sequence similarities	Belongs to the peptidase M20B family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptide metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW tripeptide aminopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	Peptidase T HAMAP-Rule MF_00550		PRO_0000185316
Sites
	Active site	80	1	By similarity
	Active site	174	1	Proton acceptor By similarity
	Metal binding	78	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 2 By similarity
	Metal binding	197	1	Zinc 1 By similarity
	Metal binding	379	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65805 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 28A88DC436056671	FASTA	408	45,820
	10 20 30 40 50 60 MKNQLIDRLT RYTTIDTQSD PKSTTTPSTE KQWDLLHLLE KELQQLGLPT DLDENGYLFA 70 80 90 100 110 120 TLESNIDADV PTVGFLAHVD TSPDFNASNV KPQIIENYDG KPYKLGNTKR VLDPKVFPEL 130 140 150 160 170 180 NSLVGHTLMV TDGTSLLGAD DKAGIVEIME AICYLQEHPE IKHGTIRIGF TPDEEIGRGP 190 200 210 220 230 240 HKFDVDRFNA DFAYTMDGSQ YGELQYESFN AAEAVITCHG VNVHPGSAKN AMVNAIRLGE 250 260 270 280 290 300 QFDSLLPDSE VPERTEGYEG FYHLMNFEGT VEKATLQYII RDHDKKQFEL RKKRILEIRD 310 320 330 340 350 360 DINAHFENYP VKVDISDQYF NMAEKILPLP HIIDIPKRVF AKLDIPANTE PIRGGTDGSQ 370 380 390 400 LSFMGLPTPN IFTGCGNFHG PYEYASIDVM EKAVQVIIGI VEDIAENH

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB56905.1.
RefSeq	NP_371267.1. NC_002758.2.
3D structure databases
ProteinModelPortal	P65805.
SMR	P65805. Positions 1-406.
ModBase	Search...
Protein-protein interaction databases
STRING	158878.SAV0743.
2D gel databases
World-2DPAGE	0002:P65805.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB56905; BAB56905; SAV0743.
GeneID	1120716.
KEGG	sav:SAV0743.
PATRIC	19562158. VBIStaAur52173_0760.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2195.
HOGENOM	HOG000032390.
KO	K01258.
OMA	YVYATIP.
ProtClustDB	PRK05469.
Enzyme and pathway databases
BioCyc	SAUR158878:GJJ5-760-MONOMER.
Family and domain databases
Gene3D	3.30.70.360. 1 hit.
HAMAP	MF_00550. Aminopeptidase_M20.
InterPro	IPR001261. ArgE/DapE_CS. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. IPR010161. Peptidase_M20B. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF037215. Peptidase_M20B. 1 hit.
SUPFAM	SSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMs	TIGR01882. peptidase-T. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPT_STAAM

Accession

Primary (citable) accession number: P65805
Secondary accession number(s): Q99VN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program