P65781 (PPNK_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	SPy_1126, M5005_Spy0848

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_0000120672

Sequences

	Sequence		Length	Mass (Da)
	P65781 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: CB69C3C3527B4981	FASTA	278	31,376
	10 20 30 40 50 60 MTQMNYTGKV KRVAIIANGK YQSKRVASKL FSVFKDDPDF YLSKKNPDIV ISIGGDGMLL 70 80 90 100 110 120 SAFHMYEKEL DKVRFVGIHT GHLGFYTDYR DFEVDKLIDN LRKDKGEQIS YPILKVAITL 130 140 150 160 170 180 DDGRVVKARA LNEATVKRIE KTMVADVIIN HVKFESFRGD GISVSTPTGS TAYNKSLGGA 190 200 210 220 230 240 VLHPTIEALQ LTEISSLNNR VFRTLGSSII IPKKDKIELV PKRLGIYTIS IDNKTYQLKN 250 260 270 VTKVEYFIDD EKIHFVSSPS HTSFWERVKD AFIGEIDS

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK34001.1. CP000017 Genomic DNA. Translation: AAZ51466.1.
RefSeq	NP_269280.1. NC_002737.1. YP_282211.1. NC_007297.1.
3D structure databases
ProteinModelPortal	P65781.
ModBase	Search...
Protein-protein interaction databases
STRING	160490.SPy_1126.
Proteomic databases
PaxDb	P65781.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK34001; AAK34001; SPy_1126. AAZ51466; AAZ51466; M5005_Spy0848.
GeneID	3572057. 901244.
KEGG	spy:SPy_1126. spz:M5005_Spy_0848.
PATRIC	19715831. VBIStrPyo79812_0980.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000275803.
KO	K00858.
OMA	RTAHAIL.
ProtClustDB	PRK04885.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_STRP1

Accession

Primary (citable) accession number: P65781
Secondary accession number(s): Q48YV6, Q99ZQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program