P65780 (PPNK_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	spr1005

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Reference proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity. HAMAP-Rule MF_00361
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00361.
Sequence similarities	Belongs to the NAD kinase family.
Sequence caution	The sequence AAK99809.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_0000120671

Sequences

	Sequence		Length	Mass (Da)
	P65780 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B9C8FC6FE604B594	FASTA	272	31,035
	10 20 30 40 50 60 MKNTGKRIDL IANRKPQSQR VLYELRDRLK RNQFILNDTN PDIVISIGGD GMLLSAFHKY 70 80 90 100 110 120 ENQLDKVRFI GLHTGHLGFY TDYRDFELDK LVTNLQLDTG ARVSYPVLNV KVFLENGEVK 130 140 150 160 170 180 IFRALNEASI RRSDRTMVAD IVINGVPFER FRGDGLTVST PTGSTAYNKS LGGAVLHPTI 190 200 210 220 230 240 EALQLTEIAS LNNRVYRTLG SSIIVPKKDK IELIPTRNDY HTISVDNSVY SFRNIERIEY 250 260 270 QIDHHKIHFV ATPSHTSFWN RVKDAFIGEV DE

References

[1]

"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007317 Genomic DNA. Translation: AAK99809.1. Different initiation.
PIR	E97997.
RefSeq	NP_358599.1. NC_003098.1.
3D structure databases
ProteinModelPortal	P65780.
ModBase	Search...
Protein-protein interaction databases
STRING	171101.spr1005.
Protocols and materials databases
DNASU	934633.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK99809; AAK99809; spr1005.
GeneID	934633.
KEGG	spr:spr1005.
PATRIC	19701952. VBIStrPne107296_1093.
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000275803.
KO	K00858.
ProtClustDB	PRK04885.
Enzyme and pathway databases
BioCyc	SPNE171101:GJC8-1017-MONOMER.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_STRR6

Accession

Primary (citable) accession number: P65780
Secondary accession number(s): Q97QV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program