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Protein

NAD kinase

Gene

nadK

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Proton acceptorUniRule annotation
Binding sitei149 – 1491NADUniRule annotation
Binding sitei151 – 1511NADUniRule annotation
Binding sitei186 – 1861NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 462NADUniRule annotation
Nucleotide bindingi122 – 1232NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1024-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:SAV1007
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269NAD kinasePRO_0000120659Add
BLAST

2D gel databases

World-2DPAGE0002:P65776.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1007.

Structurei

3D structure databases

ProteinModelPortaliP65776.
SMRiP65776. Positions 1-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiLEIHKKS.
OrthoDBiEOG6PZXDR.
PhylomeDBiP65776.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

P65776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYTILTKGD SKSNALKHKM MNYMKDFRMI EDSENPEIVI SVGGDGTLLQ
60 70 80 90 100
AFHQYSHMLS KVAFVGVHTG HLGFYADWLP HEVEKLIIEI NNSEFQVIEY
110 120 130 140 150
PLLEIIMRYN DNGYETRYLA LNEATMKTEN GSTLVVDVNL RGKHFERFRG
160 170 180 190 200
DGLCVSTPSG STAYNKALGG ALIHPSLEAM QITEIASINN RVFRTVGSPL
210 220 230 240 250
VLPKHHTCLI SPVNHDTIRM TIDHVSIKHK NVNSIQYRVA NEKVRFARFR
260
PFPFWKRVHD SFISSDEER
Length:269
Mass (Da):30,769
Last modified:October 11, 2004 - v1
Checksum:iDC51C17C51D7F7FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57169.1.
RefSeqiNP_371531.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57169; BAB57169; SAV1007.
KEGGisav:SAV1007.
PATRICi19562703. VBIStaAur52173_1032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57169.1.
RefSeqiNP_371531.1. NC_002758.2.

3D structure databases

ProteinModelPortaliP65776.
SMRiP65776. Positions 1-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1007.

2D gel databases

World-2DPAGE0002:P65776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB57169; BAB57169; SAV1007.
KEGGisav:SAV1007.
PATRICi19562703. VBIStaAur52173_1032.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiLEIHKKS.
OrthoDBiEOG6PZXDR.
PhylomeDBiP65776.

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1024-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiNADK_STAAM
AccessioniPrimary (citable) accession number: P65776
Secondary accession number(s): Q99V84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 1, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.