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Protein

NAD kinase

Gene

nadK

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. It can use ATP and other nucleoside triphosphates as a source of phosphorus. NADH cannot replace NAD as a substrate.UniRule annotation1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation1 Publication

Cofactori

a divalent metal cationUniRule annotation1 Publication

Enzyme regulationi

Allosterically inhibited by NADPH and NADH. NADPH is the primary inhibitor during aerobic growth and NADH during anaerobic growth.1 Publication

Kineticsi

  1. KM=2.1 mM for NAD1 Publication
  2. KM=2.7 mM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei73Proton acceptorUniRule annotation1
    Binding sitei158NADUniRule annotation1
    Binding sitei175NADUniRule annotation1
    Sitei175Responsible for conferring strict specificity to NADBy similarity1
    Binding sitei177NADUniRule annotation1
    Binding sitei185NAD; via carbonyl oxygenUniRule annotation1
    Binding sitei247NADUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi73 – 74NADUniRule annotation2
    Nucleotide bindingi147 – 148NADUniRule annotation2
    Nucleotide bindingi188 – 193NADUniRule annotation6

    GO - Molecular functioni

    • ATP binding Source: UniProtKB
    • metal ion binding Source: UniProtKB-HAMAP
    • NAD+ kinase activity Source: UniProtKB
    • NAD binding Source: UniProtKB

    GO - Biological processi

    • NAD metabolic process Source: InterPro
    • NADP biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Gene namesi
    Name:nadKUniRule annotation
    Ordered Locus Names:STM2683
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001206551 – 292NAD kinaseAdd BLAST292

    Proteomic databases

    PaxDbiP65774.
    PRIDEiP65774.

    Interactioni

    Subunit structurei

    Equilibrium mixture of dimer and tetramer. It is converted entirely to tetramer in the presence of the inhibitor NADPH.2 Publications

    Protein-protein interaction databases

    STRINGi99287.STM2683.

    Structurei

    Secondary structure

    1292
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 11Combined sources5
    Helixi22 – 32Combined sources11
    Beta strandi36 – 40Combined sources5
    Helixi41 – 46Combined sources6
    Helixi57 – 63Combined sources7
    Beta strandi65 – 69Combined sources5
    Helixi73 – 83Combined sources11
    Beta strandi89 – 93Combined sources5
    Beta strandi95 – 97Combined sources3
    Helixi109 – 117Combined sources9
    Beta strandi121 – 134Combined sources14
    Beta strandi138 – 140Combined sources3
    Beta strandi142 – 155Combined sources14
    Beta strandi160 – 166Combined sources7
    Beta strandi169 – 182Combined sources14
    Helixi185 – 188Combined sources4
    Helixi190 – 193Combined sources4
    Beta strandi204 – 212Combined sources9
    Beta strandi221 – 224Combined sources4
    Beta strandi229 – 233Combined sources5
    Beta strandi240 – 244Combined sources5
    Beta strandi250 – 252Combined sources3
    Beta strandi257 – 272Combined sources16
    Helixi277 – 285Combined sources9

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2AN1X-ray2.00A/B/C/D1-292[»]
    ProteinModelPortaliP65774.
    SMRiP65774.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP65774.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105F91. Bacteria.
    COG0061. LUCA.
    HOGENOMiHOG000227221.
    KOiK00858.
    OMAiKLHWHEY.
    PhylomeDBiP65774.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P65774-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CDQGYEVIVE QQIAHELQLK
    60 70 80 90 100
    NVPTGTLAEI GQQADLAVVV GGDGNMLGAA RTLARYDINV IGINRGNLGF
    110 120 130 140 150
    LTDLDPDNAL QQLSDVLEGR YISEKRFLLE AQVCQQDRQK RISTAINEVV
    160 170 180 190 200
    LHPGKVAHMI EFEVYIDETF AFSQRSDGLI ISTPTGSTAY SLSAGGPILT
    210 220 230 240 250
    PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRSDL EISCDSQIAL
    260 270 280 290
    PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF
    Length:292
    Mass (Da):32,584
    Last modified:October 11, 2004 - v1
    Checksum:i93585C83A2EEF41C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL21572.1.
    RefSeqiNP_461613.1. NC_003197.1.
    WP_001059155.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL21572; AAL21572; STM2683.
    GeneIDi1254206.
    KEGGistm:STM2683.
    PATRICi32384022. VBISalEnt20916_2828.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL21572.1.
    RefSeqiNP_461613.1. NC_003197.1.
    WP_001059155.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2AN1X-ray2.00A/B/C/D1-292[»]
    ProteinModelPortaliP65774.
    SMRiP65774.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi99287.STM2683.

    Proteomic databases

    PaxDbiP65774.
    PRIDEiP65774.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL21572; AAL21572; STM2683.
    GeneIDi1254206.
    KEGGistm:STM2683.
    PATRICi32384022. VBISalEnt20916_2828.

    Phylogenomic databases

    eggNOGiENOG4105F91. Bacteria.
    COG0061. LUCA.
    HOGENOMiHOG000227221.
    KOiK00858.
    OMAiKLHWHEY.
    PhylomeDBiP65774.

    Miscellaneous databases

    EvolutionaryTraceiP65774.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR016064. NAD/diacylglycerol_kinase.
    IPR002504. NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNADK_SALTY
    AccessioniPrimary (citable) accession number: P65774
    Secondary accession number(s): Q8XFN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: November 2, 2016
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.