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P65774

- NADK_SALTY

UniProt

P65774 - NADK_SALTY

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Protein
NAD kinase
Gene
nadK, STM2683
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. It can use ATP and other nucleoside triphosphates as a source of phosphorus. NADH can not replace NAD as a substrate.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.1 Publication

Cofactori

Divalent metal ions.1 Publication

Enzyme regulationi

Allosterically inhibited by NADPH and NADH. NADPH is the primary inhibitor during aerobic growth and NADH during anaerobic growth.1 Publication

Kineticsi

  1. KM=2.1 mM for NAD1 Publication
  2. KM=2.7 mM for ATP

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Proton acceptor By similarity
Binding sitei158 – 1581NAD By similarity
Binding sitei175 – 1751NAD By similarity
Sitei175 – 1751Responsible for conferring strict specificity to NAD By similarity
Binding sitei177 – 1771NAD By similarity
Binding sitei185 – 1851NAD; via carbonyl oxygen By similarity
Binding sitei247 – 2471NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742NAD By similarity
Nucleotide bindingi147 – 1482NAD By similarity
Nucleotide bindingi188 – 1936NAD By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. NAD binding Source: UniProtKB
  3. NAD+ kinase activity Source: UniProtKB
  4. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2698-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase (EC:2.7.1.23)
Alternative name(s):
ATP-dependent NAD kinase
Gene namesi
Name:nadK
Ordered Locus Names:STM2683
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292NAD kinaseUniRule annotation
PRO_0000120655Add
BLAST

Proteomic databases

PRIDEiP65774.

Interactioni

Subunit structurei

Equilibrium mixture of dimer and tetramer. It is converted entirely to tetramer in the presence of the inhibitor NADPH.2 Publications

Protein-protein interaction databases

STRINGi99287.STM2683.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115
Helixi22 – 3211
Beta strandi36 – 405
Helixi41 – 466
Helixi57 – 637
Beta strandi65 – 695
Helixi73 – 8311
Beta strandi89 – 935
Beta strandi95 – 973
Helixi109 – 1179
Beta strandi121 – 13414
Beta strandi142 – 15514
Beta strandi160 – 1667
Beta strandi169 – 18214
Helixi185 – 1884
Helixi190 – 1934
Beta strandi204 – 2129
Beta strandi221 – 2244
Beta strandi229 – 2335
Beta strandi240 – 2445
Beta strandi250 – 2523
Beta strandi257 – 27216
Helixi277 – 2859

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AN1X-ray2.00A/B/C/D1-292[»]
ProteinModelPortaliP65774.
SMRiP65774. Positions 4-292.

Miscellaneous databases

EvolutionaryTraceiP65774.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.

Phylogenomic databases

HOGENOMiHOG000227221.
KOiK00858.
OMAiTHEMLYH.
OrthoDBiEOG6PZXDR.
PhylomeDBiP65774.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

P65774-1 [UniParc]FASTAAdd to Basket

« Hide

MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CDQGYEVIVE QQIAHELQLK    50
NVPTGTLAEI GQQADLAVVV GGDGNMLGAA RTLARYDINV IGINRGNLGF 100
LTDLDPDNAL QQLSDVLEGR YISEKRFLLE AQVCQQDRQK RISTAINEVV 150
LHPGKVAHMI EFEVYIDETF AFSQRSDGLI ISTPTGSTAY SLSAGGPILT 200
PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRSDL EISCDSQIAL 250
PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF 292
Length:292
Mass (Da):32,584
Last modified:October 11, 2004 - v1
Checksum:i93585C83A2EEF41C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL21572.1.
RefSeqiNP_461613.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21572; AAL21572; STM2683.
GeneIDi1254206.
KEGGistm:STM2683.
PATRICi32384022. VBISalEnt20916_2828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL21572.1 .
RefSeqi NP_461613.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AN1 X-ray 2.00 A/B/C/D 1-292 [» ]
ProteinModelPortali P65774.
SMRi P65774. Positions 4-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM2683.

Proteomic databases

PRIDEi P65774.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL21572 ; AAL21572 ; STM2683 .
GeneIDi 1254206.
KEGGi stm:STM2683.
PATRICi 32384022. VBISalEnt20916_2828.

Phylogenomic databases

HOGENOMi HOG000227221.
KOi K00858.
OMAi THEMLYH.
OrthoDBi EOG6PZXDR.
PhylomeDBi P65774.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-2698-MONOMER.

Miscellaneous databases

EvolutionaryTracei P65774.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress."
    Grose J.H., Joss L., Velick S.F., Roth J.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:7601-7606(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBSTRATE SPECIFICITY, COFACTOR, SUBUNIT, NOMENCLATURE.
  3. "The crystal structure of a putative kinase from Salmonella typhimurim LT2."
    Midwest center for structural genomics (MCSG)
    Submitted (AUG-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiNADK_SALTY
AccessioniPrimary (citable) accession number: P65774
Secondary accession number(s): Q8XFN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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