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Protein

Inorganic pyrophosphatase

Gene

ppa

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 4 Mg(2+) ions per subunit. Other metal ions can support activity, but at a lower rate. Two Mg(2+) ions are required for the activation of the enzyme and are present before substrate binds, two additional Mg2+ ions form complexes with substrate and product.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Magnesium 1UniRule annotation
Metal bindingi71 – 711Magnesium 1UniRule annotation
Metal bindingi71 – 711Magnesium 2UniRule annotation
Metal bindingi103 – 1031Magnesium 1UniRule annotation

GO - Molecular functioni

  1. inorganic diphosphatase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phosphate-containing compound metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphataseUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Pyrophosphate phospho-hydrolaseUniRule annotation
Short name:
PPaseUniRule annotation
Gene namesi
Name:ppaUniRule annotation
Ordered Locus Names:BMEI0076
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 176176Inorganic pyrophosphatasePRO_0000137482Add
BLAST

Proteomic databases

PRIDEiP65744.

Interactioni

Protein-protein interaction databases

STRINGi224914.BMEI0076.

Structurei

3D structure databases

ProteinModelPortaliP65744.
SMRiP65744. Positions 3-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000236473.
KOiK01507.
OMAiKYEYDDE.
OrthoDBiEOG6NKR4X.

Family and domain databases

Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIDAISIGS NPPEDVNVII EVPVGGQPIK YEMDKKAGAL IVDRFLYTPM
60 70 80 90 100
TYPGNYGFVP HTLSEDGDPI DVLVCNTRPL IPGCVINVRP IGVLVMEDNS
110 120 130 140 150
GKDEKIIAVP SPHLTRRYEK IHDYTDMPEI TLKQIAHFFE HYKDLEPGKW
160 170
VKIGDWGDED YARKFIVEAI ERAKGK
Length:176
Mass (Da):19,822
Last modified:October 10, 2004 - v1
Checksum:i27A612793B669E31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51258.1.
PIRiAG3261.
RefSeqiNP_538994.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL51258; AAL51258; BMEI0076.
KEGGibme:BMEI0076.
PATRICi17795550. VBIBruMel146950_1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51258.1.
PIRiAG3261.
RefSeqiNP_538994.1. NC_003317.1.

3D structure databases

ProteinModelPortaliP65744.
SMRiP65744. Positions 3-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BMEI0076.

Proteomic databases

PRIDEiP65744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL51258; AAL51258; BMEI0076.
KEGGibme:BMEI0076.
PATRICi17795550. VBIBruMel146950_1048.

Phylogenomic databases

HOGENOMiHOG000236473.
KOiK01507.
OMAiKYEYDDE.
OrthoDBiEOG6NKR4X.

Family and domain databases

Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiIPYR_BRUME
AccessioniPrimary (citable) accession number: P65744
Secondary accession number(s): Q8YJK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2004
Last sequence update: October 10, 2004
Last modified: March 31, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.