Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P65744 (IPYR_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inorganic pyrophosphatase

EC=3.6.1.1
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase
Gene names
Name:ppa
Ordered Locus Names:BMEI0076
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length176 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP-Rule MF_00209

Cofactor

Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00209.

Sequence similarities

Belongs to the PPase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processphosphate-containing compound metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioninorganic diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 176176Inorganic pyrophosphatase HAMAP-Rule MF_00209
PRO_0000137482

Sites

Metal binding661Magnesium 1 By similarity
Metal binding711Magnesium 1 By similarity
Metal binding711Magnesium 2 By similarity
Metal binding1031Magnesium 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P65744 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 27A612793B669E31

FASTA17619,822
        10         20         30         40         50         60 
MNIDAISIGS NPPEDVNVII EVPVGGQPIK YEMDKKAGAL IVDRFLYTPM TYPGNYGFVP 

        70         80         90        100        110        120 
HTLSEDGDPI DVLVCNTRPL IPGCVINVRP IGVLVMEDNS GKDEKIIAVP SPHLTRRYEK 

       130        140        150        160        170 
IHDYTDMPEI TLKQIAHFFE HYKDLEPGKW VKIGDWGDED YARKFIVEAI ERAKGK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL51258.1.
PIRAG3261.
RefSeqNP_538994.1. NC_003317.1.

3D structure databases

ProteinModelPortalP65744.
SMRP65744. Positions 3-172.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0076.

Proteomic databases

PRIDEP65744.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL51258; AAL51258; BMEI0076.
GeneID1195788.
KEGGbme:BMEI0076.
PATRIC17795550. VBIBruMel146950_1048.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000236473.
KOK01507.
OMAHHISEFE.
OrthoDBEOG6NKR4X.

Family and domain databases

Gene3D3.90.80.10. 1 hit.
HAMAPMF_00209. Inorganic_PPase.
InterProIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERPTHR10286. PTHR10286. 1 hit.
PfamPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMSSF50324. SSF50324. 1 hit.
PROSITEPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIPYR_BRUME
AccessionPrimary (citable) accession number: P65744
Secondary accession number(s): Q8YJK7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names