Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P65744

- IPYR_BRUME

UniProt

P65744 - IPYR_BRUME

Protein

Inorganic pyrophosphatase

Gene

ppa

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Diphosphate + H2O = 2 phosphate.UniRule annotation

    Cofactori

    Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Magnesium 1UniRule annotation
    Metal bindingi71 – 711Magnesium 1UniRule annotation
    Metal bindingi71 – 711Magnesium 2UniRule annotation
    Metal bindingi103 – 1031Magnesium 1UniRule annotation

    GO - Molecular functioni

    1. inorganic diphosphatase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phosphate-containing compound metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inorganic pyrophosphataseUniRule annotation (EC:3.6.1.1UniRule annotation)
    Alternative name(s):
    Pyrophosphate phospho-hydrolaseUniRule annotation
    Short name:
    PPaseUniRule annotation
    Gene namesi
    Name:ppaUniRule annotation
    Ordered Locus Names:BMEI0076
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 176176Inorganic pyrophosphatasePRO_0000137482Add
    BLAST

    Proteomic databases

    PRIDEiP65744.

    Interactioni

    Protein-protein interaction databases

    STRINGi224914.BMEI0076.

    Structurei

    3D structure databases

    ProteinModelPortaliP65744.
    SMRiP65744. Positions 3-172.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000236473.
    KOiK01507.
    OMAiHHISEFE.
    OrthoDBiEOG6NKR4X.

    Family and domain databases

    Gene3Di3.90.80.10. 1 hit.
    HAMAPiMF_00209. Inorganic_PPase.
    InterProiIPR008162. Pyrophosphatase.
    [Graphical view]
    PANTHERiPTHR10286. PTHR10286. 1 hit.
    PfamiPF00719. Pyrophosphatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF50324. SSF50324. 1 hit.
    PROSITEiPS00387. PPASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P65744-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIDAISIGS NPPEDVNVII EVPVGGQPIK YEMDKKAGAL IVDRFLYTPM    50
    TYPGNYGFVP HTLSEDGDPI DVLVCNTRPL IPGCVINVRP IGVLVMEDNS 100
    GKDEKIIAVP SPHLTRRYEK IHDYTDMPEI TLKQIAHFFE HYKDLEPGKW 150
    VKIGDWGDED YARKFIVEAI ERAKGK 176
    Length:176
    Mass (Da):19,822
    Last modified:October 11, 2004 - v1
    Checksum:i27A612793B669E31
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51258.1.
    PIRiAG3261.
    RefSeqiNP_538994.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL51258; AAL51258; BMEI0076.
    GeneIDi1195788.
    KEGGibme:BMEI0076.
    PATRICi17795550. VBIBruMel146950_1048.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51258.1 .
    PIRi AG3261.
    RefSeqi NP_538994.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali P65744.
    SMRi P65744. Positions 3-172.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0076.

    Proteomic databases

    PRIDEi P65744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL51258 ; AAL51258 ; BMEI0076 .
    GeneIDi 1195788.
    KEGGi bme:BMEI0076.
    PATRICi 17795550. VBIBruMel146950_1048.

    Phylogenomic databases

    HOGENOMi HOG000236473.
    KOi K01507.
    OMAi HHISEFE.
    OrthoDBi EOG6NKR4X.

    Family and domain databases

    Gene3Di 3.90.80.10. 1 hit.
    HAMAPi MF_00209. Inorganic_PPase.
    InterProi IPR008162. Pyrophosphatase.
    [Graphical view ]
    PANTHERi PTHR10286. PTHR10286. 1 hit.
    Pfami PF00719. Pyrophosphatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50324. SSF50324. 1 hit.
    PROSITEi PS00387. PPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiIPYR_BRUME
    AccessioniPrimary (citable) accession number: P65744
    Secondary accession number(s): Q8YJK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3