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P65731 (PKNI_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase PknI

EC=2.7.11.1
Gene names
Name:pknI
Ordered Locus Names:Mb2938c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in slowing down the growth of mycobacteria within the infected host By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Manganese By similarity.

Subcellular location

Cytoplasm By similarity. Cell membrane; Single-pass membrane protein By similarity.

Post-translational modification

Autophosphorylated at serine and threonine residues By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processVirulence
   Cellular componentCell membrane
Cytoplasm
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585Serine/threonine-protein kinase PknI
PRO_0000171219

Regions

Topological domain1 – 349349Cytoplasmic Potential
Transmembrane350 – 37021Helical; Potential
Topological domain371 – 585215Extracellular Potential
Domain12 – 252241Protein kinase
Nucleotide binding18 – 269ATP By similarity

Sites

Active site1371Proton acceptor By similarity
Binding site411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P65731 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: E52EACFF4A69C3EC

FASTA58561,805
        10         20         30         40         50         60 
MALASGVTFA GYTVVRMLGC SAMGEVYLVQ HPGFPGWQAL KVLSPAMAAD DEFRRRFQRE 

        70         80         90        100        110        120 
TEVAARLFHP HILEVHDRGE FDGQLWIAMD YVDGIDATQH MADRFPAVLP VGEVLAIVTA 

       130        140        150        160        170        180 
VAGALDYAHQ RGLLHRDVNP ANVVLTSQSA GDQRILLADF GIASQPSYPA PELSAGADVD 

       190        200        210        220        230        240 
GRADQYALAL TAIHLFAGAP PVDRSHTGPL QPPKLSAFRP DLARLDGVLS RALATAPADR 

       250        260        270        280        290        300 
FGSCREFADA MNEQAGVAIA DQSSGGVDAS EVTAAAGEEA YVVDYPAYGW PEAVDCKEPS 

       310        320        330        340        350        360 
ARAPAPAAPT PQRRGSMLQS AAGVLARRLD NFSTATKAPA SPTRRRPRRI LVGAVAVLLL 

       370        380        390        400        410        420 
AGLFAVGIVI GRKTNTTATE VARPPTSGSA VPSAPTTTVA VTAPVPLDGT YRIEIQRSKQ 

       430        440        450        460        470        480 
TYDYTPTPQP PDVNTWWAFR TSCTPTECLA AATMLDDNDH TQAKTPPVRP FLMQFGEGQW 

       490        500        510        520        530        540 
KSRPETVQFP CVGPNGSPST QATTQLLALR PQPQGDLVGE MVVTVHSNEC GQQGAVIRIP 

       550        560        570        580 
AVASRSGDLP PAVTVPDPAT IPDTPDTTST ATLTPPTTTA PGPGR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248344 Genomic DNA. Translation: CAD96625.1.
RefSeqNP_856583.1. NC_002945.3.

3D structure databases

ProteinModelPortalP65731.
SMRP65731. Positions 12-246.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb2938c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD96625; CAD96625; Mb2938c.
GeneID1092167.
KEGGmbo:Mb2938c.
PATRIC18008184. VBIMycBov88188_3224.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000047111.
KOK08884.
OMANILLTHP.
OrthoDBEOG6B35XT.
ProtClustDBCLSK792157.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePKNI_MYCBO
AccessionPrimary (citable) accession number: P65731
Secondary accession number(s): Q10964
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families