P65731 (PKNI_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PknI
EC=2.7.11.1

Gene names

Name:	pknI
Ordered Locus Names:	Mb2938c

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in slowing down the growth of mycobacteria within the infected host By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Manganese By similarity.
Subcellular location	Cytoplasm By similarity. Cell membrane; Single-pass membrane protein By similarity.
Post-translational modification	Autophosphorylated at serine and threonine residues By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Serine/threonine-protein kinase PknI		PRO_0000171219
Regions
	Topological domain	1 – 349	349	Cytoplasmic Potential
	Transmembrane	350 – 370	21	Helical; Potential
	Topological domain	371 – 585	215	Extracellular Potential
	Domain	12 – 252	241	Protein kinase
	Nucleotide binding	18 – 26	9	ATP By similarity
Sites
	Active site	137	1	Proton acceptor By similarity
	Binding site	41	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65731 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: E52EACFF4A69C3EC	FASTA	585	61,805
	10 20 30 40 50 60 MALASGVTFA GYTVVRMLGC SAMGEVYLVQ HPGFPGWQAL KVLSPAMAAD DEFRRRFQRE 70 80 90 100 110 120 TEVAARLFHP HILEVHDRGE FDGQLWIAMD YVDGIDATQH MADRFPAVLP VGEVLAIVTA 130 140 150 160 170 180 VAGALDYAHQ RGLLHRDVNP ANVVLTSQSA GDQRILLADF GIASQPSYPA PELSAGADVD 190 200 210 220 230 240 GRADQYALAL TAIHLFAGAP PVDRSHTGPL QPPKLSAFRP DLARLDGVLS RALATAPADR 250 260 270 280 290 300 FGSCREFADA MNEQAGVAIA DQSSGGVDAS EVTAAAGEEA YVVDYPAYGW PEAVDCKEPS 310 320 330 340 350 360 ARAPAPAAPT PQRRGSMLQS AAGVLARRLD NFSTATKAPA SPTRRRPRRI LVGAVAVLLL 370 380 390 400 410 420 AGLFAVGIVI GRKTNTTATE VARPPTSGSA VPSAPTTTVA VTAPVPLDGT YRIEIQRSKQ 430 440 450 460 470 480 TYDYTPTPQP PDVNTWWAFR TSCTPTECLA AATMLDDNDH TQAKTPPVRP FLMQFGEGQW 490 500 510 520 530 540 KSRPETVQFP CVGPNGSPST QATTQLLALR PQPQGDLVGE MVVTVHSNEC GQQGAVIRIP 550 560 570 580 AVASRSGDLP PAVTVPDPAT IPDTPDTTST ATLTPPTTTA PGPGR

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248344 Genomic DNA. Translation: CAD96625.1.
RefSeq	NP_856583.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P65731.
SMR	P65731. Positions 12-253.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb2938c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD96625; CAD96625; Mb2938c.
GeneID	1092167.
KEGG	mbo:Mb2938c.
PATRIC	18008184. VBIMycBov88188_3224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000047111.
KO	K08884.
OMA	NILLTHP.
ProtClustDB	CLSK792157.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNI_MYCBO

Accession

Primary (citable) accession number: P65731
Secondary accession number(s): Q10964

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program