Reviewed, UniProtKB/Swiss-Prot P65722 (PHS_BRUME)

Names and origin

Protein names

Recommended name:
    Putative pterin-4-alpha-carbinolamine dehydratase
      Short name=PHS
    EC=4.2.1.96
Alternative name(s):
    4-alpha-hydroxy-tetrahydropterin dehydratase
    Pterin carbinolamine dehydratase
      Short name=PCD

Gene names

Name:	phhB
Ordered Locus Names:	BMEI1864

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	97 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin = (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O. HAMAP MF_00434
Sequence similarities	Belongs to the pterin-4-alpha-carbinolamine dehydratase family.

Ontologies

Keywords
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-alpha-hydroxytetrahydrobiopterin dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 97	97	Putative pterin-4-alpha-carbinolamine dehydratase HAMAP MF_00434		PRO_0000063076

Sequences

	Sequence		Length	Mass (Da)
	P65722-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 3176828807D408F3	FASTA	97	11,054
	10 20 30 40 50 60 MARNRLTESE MNEALRALDG WQKVDGREAI TRSFKFKDFS TAFGFMAQAA LYAEKLDHHP 70 80 90 EWFNAYNRVD VTLATHSENG VTELDIKMAR KMNAIAG

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE008917 Genomic DNA. Translation: AAL53045.1.

PIR

AB3485.

RefSeq

NP_540781.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3JST	X-ray	2.10	A/B	1-97	[»]

ModBase

Search...

Genome annotation databases

GeneID

1197575.

GenomeReviews

Gene locus BMEI1864 in contig AE008917_GR.

KEGG

bme:BMEI1864.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P65722.

OMA

SEERKTR.

Enzyme and pathway databases

BioCyc

BMEL224914:BMEI1864-MON.

BRENDA

4.2.1.96. 277959.

Family and domain databases

HAMAP

MF_00434.
[Tree]

InterPro

IPR001533. Trans/pterin_deHydtase.
[Graphical view]

Gene3D

G3DSA:3.30.1360.20. Trans_pterinDh. 1 hit.

PANTHER

PTHR12599. Trans_pterinDh. 1 hit.

Pfam

PF01329. Pterin_4a. 1 hit.
[Graphical view]

ProDom

PD007262. Trans_pterinDh. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

PHS_BRUME

Accession

Primary (citable) accession number: P65722
Secondary accession number(s): Q8YEL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)