P65700 (PGK_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	Rv1437, MT1481
ORF Names:	MTCY493.17c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00145
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from direct assay PubMed 17443846. Source: MTBBASE plasma membrane Inferred from direct assay PubMed 14532352. Source: MTBBASE
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Phosphoglycerate kinase HAMAP-Rule MF_00145		PRO_0000145973
Regions
	Nucleotide binding	360 – 363	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	63 – 66	4	Substrate binding By similarity
Sites
	Binding site	40	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	212	1	ATP By similarity
	Binding site	300	1	ATP; via carbonyl oxygen By similarity
	Binding site	331	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65700 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 0075CB4B607E65E6	FASTA	412	42,512
	10 20 30 40 50 60 MSVANLKDLL AEGVSGRGVL VRSDLNVPLD EDGTITDAGR IIASAPTLKA LLDADAKVVV 70 80 90 100 110 120 AAHLGRPKDG PDPTLSLAPV AVALGEQLGR HVQLAGDVVG ADALARAEGL TGGDILLLEN 130 140 150 160 170 180 IRFDKRETSK NDDDRRALAK QLVELVGTGG VFVSDGFGVV HRKQASVYDI ATLLPHYAGT 190 200 210 220 230 240 LVADEMRVLE QLTSSTQRPY AVVLGGSKVS DKLGVIESLA TKADSIVIGG GMCFTFLAAQ 250 260 270 280 290 300 GFSVGTSLLE DDMIEVCRGL LETYHDVLRL PVDLVVTEKF AADSPPQTVD VGAVPNGLMG 310 320 330 340 350 360 LDIGPGSIKR FSTLLSNAGT IFWNGPMGVF EFPAYAAGTR GVAEAIVAAT GKGAFSVVGG 370 380 390 400 410 GDSAAAVRAM NIPEGAFSHI STGGGASLEY LEGKTLPGIE VLSREQPTGG VL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete gene sequence of Rv1437 of Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-2016 I/05 SP." Singh A., Singh S. Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AIIMS/LM/SS/TB-2016-I/05-SP.
[2]	Singh A., Sharma P., Singh S. Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AIIMS/LM/SS/TB-1920/06/SP.
[3]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[4]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	JX987083 Genomic DNA. Translation: AGC67027.1. KC147006 Genomic DNA. Translation: AGC74190.1. AE000516 Genomic DNA. Translation: AAK45746.1. AL123456 Genomic DNA. Translation: CCP44196.1.
PIR	H70915.
RefSeq	NP_215953.1. NC_000962.3. NP_335932.1. NC_002755.2. YP_006514817.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P65700.
SMR	P65700. Positions 16-402.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv1437.
Proteomic databases
PRIDE	P65700.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK45746; AAK45746; MT1481.
GeneID	13320026. 886636. 924491.
KEGG	mtc:MT1481. mtu:Rv1437.
PATRIC	18125020. VBIMycTub22151_1625.
Organism-specific databases
TubercuList	Rv1437.
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227107.
KO	K00927.
OMA	EKKNDEA.
ProtClustDB	PRK00073.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_MYCTU

Accession

Primary (citable) accession number: P65700
Secondary accession number(s): L0T6N0, O06821

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program