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P65694

- PFKA_STAAM

UniProt

P65694 - PFKA_STAAM

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, pfk, SAV1698
Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Active sitei129 – 1291Proton acceptor By similarity
Binding sitei156 – 1561Allosteric activator ADP By similarity
Binding sitei164 – 1641Substrate; shared with dimeric partner By similarity
Binding sitei213 – 2131Allosteric activator ADP By similarity
Binding sitei224 – 2241Substrate By similarity
Binding sitei245 – 2451Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarity
Nucleotide bindingi102 – 1054ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1714-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
Ordered Locus Names:SAV1698
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000111977Add
BLAST

2D gel databases

World-2DPAGE0002:P65694.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV1698.

Structurei

3D structure databases

ProteinModelPortaliP65694.
SMRiP65694. Positions 1-322.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni127 – 1293Substrate binding By similarity
Regioni171 – 1733Substrate binding By similarity
Regioni187 – 1893Allosteric activator ADP binding By similarity
Regioni215 – 2173Allosteric activator ADP binding By similarity
Regioni251 – 2544Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65694-1 [UniParc]FASTAAdd to Basket

« Hide

MKKIAVLTSG GDSPGMNAAV RAVVRTAIYN EIEVYGVYHG YQGLLNDDIH    50
KLELGSVGDT IQRGGTFLYS ARCPEFKEQE VRKVAIENLR KRGIEGLVVI 100
GGDGSYRGAQ RISEECKEIQ TIGIPGTIDN DINGTDFTIG FDTALNTIIG 150
LVDKIRDTAS SHARTFIIEA MGRDCGDLAL WAGLSVGAET IVVPEVKTDI 200
KEIADKIEQG IKRGKKHSIV LVAEGCMTAQ DCQKELSQYI NVDNRVSVLG 250
HVQRGGSPTG ADRVLASRLG GYAVDLLMQG ETAKGVGIKN NKIVATSFDE 300
IFDGKDHKFD YSLYELANKL SI 322
Length:322
Mass (Da):34,840
Last modified:October 11, 2004 - v1
Checksum:i1FAE41C0BA7CA7F1
GO

Sequence cautioni

The sequence BAB57860.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57860.1. Different initiation.
RefSeqiNP_372222.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57860; BAB57860; SAV1698.
GeneIDi1121673.
KEGGisav:SAV1698.
PATRICi19564148. VBIStaAur52173_1752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57860.1 . Different initiation.
RefSeqi NP_372222.1. NC_002758.2.

3D structure databases

ProteinModelPortali P65694.
SMRi P65694. Positions 1-322.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 158878.SAV1698.

2D gel databases

World-2DPAGE 0002:P65694.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB57860 ; BAB57860 ; SAV1698 .
GeneIDi 1121673.
KEGGi sav:SAV1698.
PATRICi 19564148. VBIStaAur52173_1752.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci SAUR158878:GJJ5-1714-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiPFKA_STAAM
AccessioniPrimary (citable) accession number: P65694
Secondary accession number(s): Q99TG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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