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P65691

- PFKA_MYCBO

UniProt

P65691 - PFKA_MYCBO

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, Mb3035c
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogen By similarity
Metal bindingi104 – 1041Magnesium; catalytic By similarity
Sitei105 – 1051Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei128 – 1281Proton acceptor By similarity
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei267 – 2671Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATP By similarity
Nucleotide bindingi103 – 1064ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:Mb3035c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000111969Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi233413.Mb3035c.

Structurei

3D structure databases

ProteinModelPortaliP65691.
SMRiP65691. Positions 2-335.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate binding By similarity
Regioni170 – 1723Substrate binding By similarity
Regioni273 – 2764Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGDFGRM.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65691-1 [UniParc]FASTAAdd to Basket

« Hide

MRIGVLTGGG DCPGLNAVIR AVVRTCHARY GSSVVGFQNG FRGLLENRRV    50
QLHNDDRNDR LLAKGGTMLG TARVHPDKLR AGLPQIMQTL DDNGIDVLIP 100
IGGEGTLTAA SWLSEENVPV VGVPKTIDND IDCTDVTFGH DTALTVATEA 150
IDRLHSTAES HERVMLVEVM GRHAGWIALN AGLASGAHMT LIPEQPFDIE 200
EVCRLVKGRF QRGDSHFICV VAEGAKPAPG TIMLREGGLD EFGHERFTGV 250
AAQLAVEVEK RINKDVRVTV LGHIQRGGTP TAYDRVLATR FGVNAADAAH 300
AGEYGQMVTL RGQDIGRVPL ADAVRKLKLV PQSRYDDAAA FFG 343
Length:343
Mass (Da):36,880
Last modified:October 11, 2004 - v1
Checksum:i826E5FF16A6ECABA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44303.1.
RefSeqiNP_856680.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96722; CAD96722; Mb3035c.
GeneIDi1093228.
KEGGimbo:Mb3035c.
PATRICi18008410. VBIMycBov88188_3335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44303.1 .
RefSeqi NP_856680.1. NC_002945.3.

3D structure databases

ProteinModelPortali P65691.
SMRi P65691. Positions 2-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb3035c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96722 ; CAD96722 ; Mb3035c .
GeneIDi 1093228.
KEGGi mbo:Mb3035c.
PATRICi 18008410. VBIMycBov88188_3335.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi RGDFGRM.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPFKA_MYCBO
AccessioniPrimary (citable) accession number: P65691
Secondary accession number(s): O53257, X2BMF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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