Reviewed, UniProtKB/Swiss-Prot P65670 (CYSD_MYCTU)

Names and origin

Protein names

Recommended name:
    Sulfate adenylyltransferase subunit 2
    EC=2.7.7.4
Alternative name(s):
    Sulfate adenylate transferase
      Short name=SAT
    ATP-sulfurylase small subunit

Gene names

Name:	cysD
Ordered Locus Names:	Rv1285, MT1323
ORF Names:	MTCY373.04

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of cysD, the smaller subunit, and cysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000100667

Sequences

	Sequence		Length	Mass (Da)
	P65670-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 75D965D35F1EC284	FASTA	309	34,889
	10 20 30 40 50 60 MTSDVTVGPA PGQYQLSHLR LLEAEAIHVI REVAAEFERP VLLFSGGKDS IVMLHLALKA 70 80 90 100 110 120 FRPGRLPFPV MHVDTGHNFD EVIATRDELV AAAGVRLVVA SVQDDIDAGR VVETIPSRNP 130 140 150 160 170 180 IQTVTLLRAI RENQFDAAFG GARRDEEKAR AKERVFSFRD EFGQWDPKAQ RPELWNLYNG 190 200 210 220 230 240 RHHKGEHIRV FPLSNWTEFD IWSYIGAEQV RLPSIYFAHR RKVFQRDGML LAVHRHMQPR 250 260 270 280 290 300 ADEPVFEATV RFRTVGDVTC TGCVESSAST VAEVIAETAV ARLTERGATR ADDRISEAGM EDRKRQGYF

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842576 Genomic DNA. Translation: CAA97751.1. Different initiation. AE000516 Genomic DNA. Translation: AAK45584.1.
PIR	A70772.
RefSeq	NP_215801.1. NP_335770.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	886979. 924748.
GenomeReviews	Gene locus MT1323 in contig AE000516_GR. Gene locus Rv1285 in contig AL123456_GR.
KEGG	mtc:MT1323. mtu:Rv1285.
TIGR	MT1323.
Organism-specific databases
TubercuList	Rv1285.
Phylogenomic databases
HOGENOM	P65670.
OMA	P65670. NYPEVIQ.
Enzyme and pathway databases
BRENDA	2.7.7.4. 809.
Family and domain databases
HAMAP	MF_00064. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_MYCTU

Accession

Primary (citable) accession number: P65670
Secondary accession number(s): Q10599

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)