Reviewed, UniProtKB/Swiss-Prot P65669 (CYSH_MYCBO)

Names and origin

Protein names

Recommended name:
    Probable phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	Mb2413

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Probable phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100634

Sequences

	Sequence		Length	Mass (Da)
	P65669-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 554F2E3204452C55	FASTA	254	27,423
	10 20 30 40 50 60 MSGETTRLTE PQLRELAARG AAELDGATAT DMLRWTDETF GDIGGAGGGV SGHRGWTTCN 70 80 90 100 110 120 YVVASNMADA VLVDLAAKVR PGVPVIFLDT GYHFVETIGT RDAIESVYDV RVLNVTPEHT 130 140 150 160 170 180 VAEQDELLGK DLFARNPHEC CRLRKVVPLG KTLRGYSAWV TGLRRVDAPT RANAPLVSFD 190 200 210 220 230 240 ETFKLVKVNP LAAWTDQDVQ EYIADNDVLV NPLVREGYPS IGCAPCTAKP AEGADPRSGR 250 WQGLAKTECG LHAS

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248342 Genomic DNA. Translation: CAD97274.1.
RefSeq	NP_856062.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1094304.
GenomeReviews	Gene locus Mb2413 in contig BX248333_GR.
KEGG	mbo:Mb2413.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P65669.
OMA	P65669. FEETLAY.
Enzyme and pathway databases
BRENDA	1.8.4.8. 3091.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. cysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_MYCBO

Accession

Primary (citable) accession number: P65669
Secondary accession number(s): P71752

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)