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Protein

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex

Gene

dlaT

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.By similarity

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.

Cofactori

(R)-lipoateBy similarityNote: Binds 2 lipoyl cofactors covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei523By similarity1
Active sitei527By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycolysis

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-2306-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2
Short name:
PDH component E2
Gene namesi
Name:dlaT
Synonyms:sucB
Ordered Locus Names:BQ2027_MB2238
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001622671 – 553Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complexAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43N6-lipoyllysinePROSITE-ProRule annotation1
Modified residuei162N6-lipoyllysinePROSITE-ProRule annotation1

Interactioni

Subunit structurei

Forms a 24-polypeptide structural core with octahedral symmetry. Part of the PDH complex, consisting of multiple copies of AceE (E1), DlaT (E2) and Lpd (E3).By similarity

Structurei

3D structure databases

ProteinModelPortaliP65634
SMRiP65634
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 77Lipoyl-binding 1PROSITE-ProRule annotationAdd BLAST76
Domaini121 – 196Lipoyl-binding 2PROSITE-ProRule annotationAdd BLAST76
Domaini243 – 280Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated

Keywords - Domaini

Lipoyl, Repeat

Phylogenomic databases

HOGENOMiHOG000281564
OMAiMKVPSPG

Family and domain databases

Gene3Di3.30.559.10, 1 hit
4.10.320.10, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR001078 2-oxoacid_DH_actylTfrase
IPR014276 2-oxoglutarate_DH_E2
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 2 hits
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 2 hits
TIGRFAMsiTIGR02927 SucB_Actino, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 2 hits
PS00189 LIPOYL, 2 hits
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

P65634-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSVQMPAL GESVTEGTVT RWLKQEGDTV ELDEPLVEVS TDKVDTEIPS
60 70 80 90 100
PAAGVLTKII AQEDDTVEVG GELAVIGDAK DAGEAAAPAP EKVPAAQPES
110 120 130 140 150
KPAPEPPPVQ PTSGAPAGGD AKPVLMPELG ESVTEGTVIR WLKKIGDSVQ
160 170 180 190 200
VDEPLVEVST DKVDTEIPSP VAGVLVSISA DEDATVPVGG ELARIGVAAD
210 220 230 240 250
IGAAPAPKPA PKPVPEPAPT PKAEPAPSPP AAQPAGAAEG APYVTPLVRK
260 270 280 290 300
LASENNIDLA GVTGTGVGGR IRKQDVLAAA EQKKRAKAPA PAAQAAAAPA
310 320 330 340 350
PKAPPAPAPA LAHLRGTTQK ASRIRQITAN KTRESLQATA QLTQTHEVDM
360 370 380 390 400
TKIVGLRARA KAAFAEREGV NLTFLPFFAK AVIDALKIHP NINASYNEDT
410 420 430 440 450
KEITYYDAEH LGFAVDTEQG LLSPVIHDAG DLSLAGLARA IADIAARARS
460 470 480 490 500
GNLKPDELSG GTFTITNIGS QGALFDTPIL VPPQAAMLGT GAIVKRPRVV
510 520 530 540 550
VDASGNESIG VRSVCYLPLT YDHRLIDGAD AGRFLTTIKH RLEEGAFEAD

LGL
Length:553
Mass (Da):57,088
Last modified:October 11, 2004 - v1
Checksum:i54B6E70D23B804A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU00846.1
RefSeqiNP_855887.1, NC_002945.3
WP_003411450.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO43492; CDO43492; Mb2238
PATRICifig|233413.5.peg.2454

Similar proteinsi

Entry informationi

Entry nameiODP2_MYCBO
AccessioniPrimary (citable) accession number: P65634
Secondary accession number(s): A0A1R3Y0P9, Q10381, X2BJM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health