P65611 (PYRB_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	BMEII0670

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP-Rule MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP-Rule MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway 'de novo' pyrimidine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Aspartate carbamoyltransferase HAMAP-Rule MF_00001		PRO_0000113108

Sequences

	Sequence		Length	Mass (Da)
	P65611 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 81B6208851B9521B	FASTA	322	34,802
	10 20 30 40 50 60 MTNQTVSPLF PHRHLLGIKG LSPLDILCLL DLADQEIAVS RQPEKKKSVL RGRTQINLFF 70 80 90 100 110 120 EASTRTQSSF ELAGKRLGAD VMNMSVGNSS VKKGETLIDT AMTLNAMQPD ILVIRHASAG 130 140 150 160 170 180 AAALLAQKVG CSVVNAGDGA HEHPTQALLD ALTIRRAKGQ IENLIVAICG DVLHSRVARS 190 200 210 220 230 240 NILLLNALGA RVRVVAPSTL LPAGMADMSV EVFNSMEEGL KDADVVMMLR LQRERMAGSF 250 260 270 280 290 300 VPSVREYFHF YGLDREKLKF AKPDALVMHP GPMNRGVEIA SDVADGPQSV IQQQVEMGVA 310 320 VRMAVMEALL DPRRNPGNGE PA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL53912.1.
PIR	AE3593.
RefSeq	NP_541648.1. NC_003318.1.
3D structure databases
ProteinModelPortal	P65611.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEII0670.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL53912; AAL53912; BMEII0670.
GeneID	1198442.
KEGG	bme:BMEII0670.
PATRIC	17799738. VBIBruMel146950_2205.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000022685.
KO	K00609.
OMA	HERHAGD.
ProtClustDB	PRK00856.
Enzyme and pathway databases
UniPathway	UPA00070; UER00116.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr.
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR002082. Asp_carbamoyltransf. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BRUME

Accession

Primary (citable) accession number: P65611
Secondary accession number(s): Q8YC62

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program