Reviewed, UniProtKB/Swiss-Prot P65609 (OTCC_STRP3)

Names and origin

Protein names

Recommended name:
    Ornithine carbamoyltransferase, catabolic
      Short name=OTCase
    EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	SpyM3_1194, SPs0668

Organism

Streptococcus pyogenes serotype M3 [Complete proteome] [HAMAP]

Taxonomic identifier

301448 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 337	336	Ornithine carbamoyltransferase, catabolic HAMAP MF_01109		PRO_0000113039
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	271 – 274	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65609-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A215B1EF1E0E0C43	FASTA	337	37,897
	10 20 30 40 50 60 MTQVFQGRSF LAEKDFTRAE LEYLIDFSAH LKDLKKRGVP HHYLEGKNIA LLFEKTSTRT 70 80 90 100 110 120 RAAFTTAAID LGAHPEYLGA NDIQLGKKES TEDTAKVLGR MFDGIEFRGF SQRMVEELAE 130 140 150 160 170 180 FSGVPVWNGL TDEWHPTQML ADYLTVKENF GKLEGLTLVY CGDGRNNVAN SLLVTGAILG 190 200 210 220 230 240 VNVHIFSPKE LFPEEEIVTL AEGYAKESGA RILITEDADE AVKGADVLYT DVWVSMGEED 250 260 270 280 290 300 KFKERVELLQ PYQVNMDLVQ KAGNDKLIFL HCLPAFHDTN TVYGKDVAEK FGVKEMEVTD 310 320 330 EVFRSKYARH FDQAENRMHT IKAVMAATLG NLFIPKV

References

[1]

"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence." Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed: 12122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-595 / MGAS315 / Serotype M3.

[2]

"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed: 12799345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1 / Serotype M3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014074 Genomic DNA. Translation: AAM79801.1. BA000034 Genomic DNA. Translation: BAC63763.1.
RefSeq	NP_664998.1. NP_801930.1.
3D structure databases
HSSP	HSSP built from PDB template 1AKM based on UniProtKB P04391.
ModBase	Search...
Genome annotation databases
GeneID	1009509. 1066947.
GenomeReviews	Gene locus SpyM3_1194 in contig AE014074_GR. Gene locus SPs0668 in contig BA000034_GR.
KEGG	spg:SpyM3_1194. sps:SPs0668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P65609.
OMA	FRSKHSK.
Enzyme and pathway databases
BioCyc	SPYO193567:SPS0668-MON. SPYO198466:SPYM3_1194-MON.
Family and domain databases
HAMAP	MF_01109. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTCC_STRP3

Accession

Primary (citable) accession number: P65609
Secondary accession number(s): Q8P052

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)