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P65575 (NUOB_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit B
EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit B
NDH-1 subunit B
Gene names
Name:nuoB
Ordered Locus Names:Rv3146, MT3234
ORF Names:MTCY03A2.12c
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length184 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01356

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP MF_01356

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01356

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_01356.

Sequence similarities

Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
NAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

NADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 184184NADH-quinone oxidoreductase subunit B HAMAP MF_01356
PRO_0000118773

Sites

Metal binding371Iron-sulfur (4Fe-4S) Potential
Metal binding381Iron-sulfur (4Fe-4S) Potential
Metal binding1031Iron-sulfur (4Fe-4S) Potential
Metal binding1321Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence LengthMass (Da)Tools
P65575 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 9E8C05D2A8BC18A0

FASTA18420,219
        10         20         30         40         50         60 
MGLEEQLPGG ILLSTVEKVA GYVRKNSLWP ATFGLACCAI EMMATAGPRF DIARFGMERF 

        70         80         90        100        110        120 
SATPRQADLM IVAGRVSQKM APVLRQIYDQ MAEPKWVLAM GVCASSGGMF NNYAIVQGVD 

       130        140        150        160        170        180 
HVVPVDIYLP GCPPRPEMLL HAILKLHEKI QQMPLGINRE RAIAEAEEAA LLARPTIEMR 


GLLR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842582 Genomic DNA. Translation: CAB06270.1.
AE000516 Genomic DNA. Translation: AAK47573.1.
PIRC70647.
RefSeqNP_217662.1. NC_000962.2.
NP_337759.1. NC_002755.2.

3D structure databases

ProteinModelPortalP65575.
SMRP65575. Positions 10-151.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000002046; EBMYCP00000002046; EBMYCG00000002044.
EBMYCT00000070552; EBMYCP00000068611; EBMYCG00000070547.
GeneID888791.
923386.
GenomeReviewsGene locus MT3234 in contig AE000516_GR.
Gene locus Rv3146 in contig AL123456_GR.
KEGGmtc:MT3234.
mtu:Rv3146.
PATRIC18128874. VBIMycTub22151_3534.
TIGRMT3234.

Organism-specific databases

TubercuListRv3146.

Phylogenomic databases

GeneTreeEBGT00050000016353.
HOGENOMHBG553221.
OMAQNKIRRT.
PhylomeDBP65575.
ProtClustDBPRK06411.

Family and domain databases

HAMAPMF_01356. NDH1_NuoB.
[Tree]
InterProIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
IPR014406. NiFe-hyd_3_ssu/Q_oxred_NuoB.
[Graphical view]
Gene3DG3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KOK00331.
PANTHERPTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
PfamPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsTIGR01957. NuoB_fam. 1 hit.
PROSITEPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOB_MYCTU
AccessionPrimary (citable) accession number: P65575
Secondary accession number(s): P95180
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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