Reviewed, UniProtKB/Swiss-Prot P65567 (NUOF_MYCTU)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	Rv3150, MT3238
ORF Names:	MTCY03A2.08c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	NADH-quinone oxidoreductase subunit F		PRO_0000118574
Regions
	Nucleotide binding	61 – 70	10	NAD By similarity
	Nucleotide binding	177 – 224	48	FMN By similarity
Sites
	Metal binding	353	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	356	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	359	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	399	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P65567-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C02A660F43842926	FASTA	445	48,102
	10 20 30 40 50 60 MTTQATPLTP VISRHWDDPE SWTLATYQRH DRYRGYQALQ KALTMPPDDV ISIVKDSGLR 70 80 90 100 110 120 GRGGAGFATG TKWSFIPQGD TGAAAKPHYL VVNADESEPG TCKDIPLMLA TPHVLIEGVI 130 140 150 160 170 180 IAAYAIRAHH AFVYVRGEVV PVLRRLHNAV AEAYAAGFLG RNIGGSGFDL ELVVHAGAGA 190 200 210 220 230 240 YICGEETALL DSLEGRRGQP RLRPPFPAVA GLYGCPTVIN NVETIASVPS IILGGIDWFR 250 260 270 280 290 300 SMGSEKSPGF TLYSLSGHVT RPGQYEAPLG ITLRELLDYA GGVRAGHRLK FWTPGGSSTP 310 320 330 340 350 360 LLTDEHLDVP LDYEGVGAAG SMLGTKALEI FDETTCVVRA VRRWTEFYKH ESCGKCTPCR 370 380 390 400 410 420 EGTFWLDKIY ERLETGRGSH EDIDKLLDIS DSILGKSFCA LGDGAASPVM SSIKHFRDEY 430 440 LAHVEGGGCP FDPRDSMLVA NGVDA

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842582 Genomic DNA. Translation: CAB06289.1. AE000516 Genomic DNA. Translation: AAK47577.1.
PIR	G70647.
RefSeq	NP_217666.1. NP_337763.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	888854. 923375.
GenomeReviews	Gene locus MT3238 in contig AE000516_GR. Gene locus Rv3150 in contig AL123456_GR.
KEGG	mtc:MT3238. mtu:Rv3150.
TIGR	MT3238.
Organism-specific databases
TubercuList	Rv3150.
Phylogenomic databases
HOGENOM	P65567.
OMA	P65567. AMPVRAM.
Enzyme and pathway databases
BRENDA	1.6.99.5. 809.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_MYCTU

Accession

Primary (citable) accession number: P65567
Secondary accession number(s): P95176

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)