NADH-quinone oxidoreductase subunit F - Mycobacterium tuberculosis

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P65567 (NUOF_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit F
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	Rv3150, MT3238
ORF Names:	MTCY03A2.08c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD⁺ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 445

445

NADH-quinone oxidoreductase subunit F

PRO_0000118574

Regions

Nucleotide binding

61 – 70

NAD By similarity

Nucleotide binding

177 – 224

FMN By similarity

Sites

Metal binding

353

Iron-sulfur (4Fe-4S) Potential

Metal binding

356

Iron-sulfur (4Fe-4S) Potential

Metal binding

359

Iron-sulfur (4Fe-4S) Potential

Metal binding

399

Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P65567 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: C02A660F43842926
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FASTA

445

48,102

        10         20         30         40         50         60 
MTTQATPLTP VISRHWDDPE SWTLATYQRH DRYRGYQALQ KALTMPPDDV ISIVKDSGLR 

        70         80         90        100        110        120 
GRGGAGFATG TKWSFIPQGD TGAAAKPHYL VVNADESEPG TCKDIPLMLA TPHVLIEGVI 

       130        140        150        160        170        180 
IAAYAIRAHH AFVYVRGEVV PVLRRLHNAV AEAYAAGFLG RNIGGSGFDL ELVVHAGAGA 

       190        200        210        220        230        240 
YICGEETALL DSLEGRRGQP RLRPPFPAVA GLYGCPTVIN NVETIASVPS IILGGIDWFR 

       250        260        270        280        290        300 
SMGSEKSPGF TLYSLSGHVT RPGQYEAPLG ITLRELLDYA GGVRAGHRLK FWTPGGSSTP 

       310        320        330        340        350        360 
LLTDEHLDVP LDYEGVGAAG SMLGTKALEI FDETTCVVRA VRRWTEFYKH ESCGKCTPCR 

       370        380        390        400        410        420 
EGTFWLDKIY ERLETGRGSH EDIDKLLDIS DSILGKSFCA LGDGAASPVM SSIKHFRDEY 

       430        440 
LAHVEGGGCP FDPRDSMLVA NGVDA

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References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.

Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R.

Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842582 Genomic DNA. Translation: CAB06289.1. AE000516 Genomic DNA. Translation: AAK47577.1.
PIR	G70647.
RefSeq	NP_217666.1. NC_000962.2. NP_337763.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P65567.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000521; EBMYCP00000000521; EBMYCG00000000521. EBMYCT00000072580; EBMYCP00000070639; EBMYCG00000072575.
GeneID	888854. 923375.
GenomeReviews	Gene locus MT3238 in contig AE000516_GR. Gene locus Rv3150 in contig AL123456_GR.
KEGG	mtc:MT3238. mtu:Rv3150.
PATRIC	18128882. VBIMycTub22151_3538.
TIGR	MT3238.
Organism-specific databases
TubercuList	Rv3150.
Phylogenomic databases
GeneTree	EBGT00050000016197.
HOGENOM	HBG669338.
OMA	KHAGGVT.
PhylomeDB	P65567.
ProtClustDB	CLSK872146.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
KO	K00335.
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. NuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_MYCTU

Accession

Primary (citable) accession number: P65567
Secondary accession number(s): P95176

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents