Reviewed, UniProtKB/Swiss-Prot P65529 (FPRB_MYCBO)

Names and origin

Protein names

Recommended name:
    Probable ferredoxin/ferredoxin--NADP reductase
      Short name=FNR
    EC=1.18.1.2

Gene names

Name:	fprB
Ordered Locus Names:	Mb0910

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	Binds 1 or 2 4Fe-4S clusters. FAD.
Sequence similarities	In the C-terminal section; belongs to the ferredoxin--NADP reductase family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S FAD Flavoprotein Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Probable ferredoxin/ferredoxin--NADP reductase		PRO_0000167675
Regions
	Domain	2 – 29	28	4Fe-4S ferredoxin-type 1
	Domain	37 – 66	30	4Fe-4S ferredoxin-type 2
	Nucleotide binding	258 – 261	4	NADP By similarity
	Nucleotide binding	302 – 303	2	NADP By similarity
	Nucleotide binding	463 – 465	3	FAD By similarity
	Region	115 – 575	461	Ferredoxin--NADP reductase
Sites
	Metal binding	9	1	Iron-sulfur 1 By similarity
	Metal binding	15	1	Iron-sulfur 1 By similarity
	Metal binding	19	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	46	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	52	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	56	1	Iron-sulfur 1 By similarity
	Binding site	123	1	FAD; via amide nitrogen By similarity
	Binding site	143	1	FAD By similarity
	Binding site	151	1	FAD; via amide nitrogen By similarity
	Binding site	187	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	213	1	NADP By similarity
	Binding site	314	1	NADP By similarity
	Binding site	456	1	FAD; via amide nitrogen By similarity
	Binding site	463	1	NADP; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65529-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 170C58599872A810	FASTA	575	61,337
	10 20 30 40 50 60 MPHVITQSCC NDASCVFACP VNCIHPTPDE PGFATSEMLY IDPVACVDCG ACVTACPVSA 70 80 90 100 110 120 IAPNTRLDFE QLPFVEINAS YYPKRPAGVK LAPTSKLAPV TPAAEVRVRR QPLTVAVVGS 130 140 150 160 170 180 GPAAMYAADE LLVQQGVQVN VFEKLPTPYG LVRSGVAPDH QNTKRVTRLF DRIAGHRRFR 190 200 210 220 230 240 FYLNVEIGKH LGHAELLAHH HAVLYAVGAP DDRRLTIDGM GLPGTGTATE LVAWLNGHPD 250 260 270 280 290 300 FNDLPVDLSH ERVVIIGNGN VALDVARVLA ADPHELAATD IADHALSALR NSAVREVVVA 310 320 330 340 350 360 ARRGPAHSAF TLPELIGLTA GADVVLDPGD HQRVLDDLAI VADPLTRNKL EILSTLGDGS 370 380 390 400 410 420 APARRVGRPR IRLAYRLTPR RVLGQRRAGG VQFSVTGTDE LRQLDAGLVL TSIGYRGKPI 430 440 450 460 470 480 PDLPFDEQAA LVPNDGGRVI DPGTGEPVPG AYVAGWIKRG PTGFIGTNKS CSMQTVQALV 490 500 510 520 530 540 ADFNDGRLTD PVATPTALDQ LVQARQPQAI GCAGWRAIDA AEIARGSADG RVRNKFTDVA 550 560 570 EMLAAATSAP KEPLRRRVLA RLRDLGQPIV LTVPL

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248336 Genomic DNA. Translation: CAD93771.1.
RefSeq	NP_854567.1.
3D structure databases
HSSP	HSSP built from PDB template 1H98 based on UniProtKB P03942.
ModBase	Search...
Genome annotation databases
GeneID	1092796.
GenomeReviews	Gene locus Mb0910 in contig BX248333_GR.
KEGG	mbo:Mb0910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P65529.
OMA	P65529. DRIKEVP.
Enzyme and pathway databases
BRENDA	1.18.1.2. 3091.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR000759. Adrndx_reductase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00037. Fer4. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FPRB_MYCBO

Accession

Primary (citable) accession number: P65529
Secondary accession number(s): Q10547

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)