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Protein

Putative N-acetylmannosamine-6-phosphate 2-epimerase

Gene

nanE

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P).UniRule annotation

Catalytic activityi

N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate.UniRule annotation

Pathwayi: N-acetylneuraminate degradation

This protein is involved in step 3 of the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. N-acetylneuraminate lyase (nanA)
  2. no protein annotated in this organism
  3. Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE)
  4. no protein annotated in this organism
  5. Glucosamine-6-phosphate deaminase (nagB)
This subpathway is part of the pathway N-acetylneuraminate degradation, which is itself part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate, the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-316-MONOMER.
UniPathwayiUPA00629; UER00682.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative N-acetylmannosamine-6-phosphate 2-epimeraseUniRule annotation (EC:5.1.3.9UniRule annotation)
Alternative name(s):
ManNAc-6-P epimeraseUniRule annotation
Gene namesi
Name:nanEUniRule annotation
Ordered Locus Names:SA0307
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Putative N-acetylmannosamine-6-phosphate 2-epimerasePRO_0000179799Add
BLAST

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi21 – 3414Combined sources
Beta strandi37 – 437Combined sources
Helixi44 – 5310Combined sources
Beta strandi58 – 614Combined sources
Helixi77 – 8610Combined sources
Beta strandi89 – 946Combined sources
Beta strandi101 – 1033Combined sources
Helixi105 – 11511Combined sources
Beta strandi119 – 1246Combined sources
Helixi128 – 1369Combined sources
Beta strandi140 – 1434Combined sources
Turni145 – 1484Combined sources
Helixi160 – 17213Combined sources
Beta strandi175 – 1828Combined sources
Helixi186 – 1949Combined sources
Beta strandi198 – 2025Combined sources
Helixi204 – 2074Combined sources
Helixi209 – 21810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y0EX-ray1.95A/B1-222[»]
ProteinModelPortaliP65517.
SMRiP65517. Positions 1-221.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP65517.

Family & Domainsi

Sequence similaritiesi

Belongs to the NanE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000236669.
OMAiITRPMEI.
OrthoDBiEOG6NSGKX.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01235. ManNAc6P_epimer.
InterProiIPR013785. Aldolase_TIM.
IPR007260. NanE.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF04131. NanE. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequencei

Sequence statusi: Complete.

P65517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPHGLIVSC QALADEPLHS SFIMSKMALA AYEGGAVGIR ANTKEDILAI
60 70 80 90 100
KETVDLPVIG IVKRDYDHSD VFITATSKEV DELIESQCEV IALDATLQQR
110 120 130 140 150
PKETLDELVS YIRTHAPNVE IMADIATVEE AKNAARLGFD YIGTTLHGYT
160 170 180 190 200
SYTQGQLLYQ NDFQFLKDVL QSVDAKVIAE GNVITPDMYK RVMDLGVHCS
210 220
VVGGAITRPK EITKRFVQVM ED
Length:222
Mass (Da):24,505
Last modified:October 11, 2004 - v1
Checksum:i2977C9D2E628BD2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB41531.1.
PIRiH89796.
RefSeqiWP_000936718.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB41531; BAB41531; BAB41531.
PATRICi19572666. VBIStaAur116463_0322.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB41531.1.
PIRiH89796.
RefSeqiWP_000936718.1. NC_002745.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y0EX-ray1.95A/B1-222[»]
ProteinModelPortaliP65517.
SMRiP65517. Positions 1-221.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB41531; BAB41531; BAB41531.
PATRICi19572666. VBIStaAur116463_0322.

Phylogenomic databases

HOGENOMiHOG000236669.
OMAiITRPMEI.
OrthoDBiEOG6NSGKX.

Enzyme and pathway databases

UniPathwayiUPA00629; UER00682.
BioCyciSAUR158879:GJCB-316-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP65517.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01235. ManNAc6P_epimer.
InterProiIPR013785. Aldolase_TIM.
IPR007260. NanE.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF04131. NanE. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: N315.

Entry informationi

Entry nameiNANE_STAAN
AccessioniPrimary (citable) accession number: P65517
Secondary accession number(s): Q99WQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 16, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.