Reviewed, UniProtKB/Swiss-Prot P65499 (NADB_MYCTU)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	Rv1595, MT1631
ORF Names:	MTCY336.09c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	L-aspartate oxidase		PRO_0000184389
Regions
	Nucleotide binding	13 – 27	15	FAD Potential
Sites
	Active site	233	1	By similarity
	Active site	254	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65499-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5494D354B107E5DA	FASTA	527	53,785
	10 20 30 40 50 60 MAGPAWRDAA DVVVIGTGVA GLAAALAADR AGRSVVVLSK AAQTHVTATH YAQGGIAVVL 70 80 90 100 110 120 PDNDDSVDAH VADTLAAGAG LCDPDAVYSI VADGYRAVTD LVGAGARLDE SVPGRWALTR 130 140 150 160 170 180 EGGHSRRRIV HAGGDATGAE VQRALQDAAG MLDIRTGHVA LRVLHDGTAV TGLLVVRPDG 190 200 210 220 230 240 CGIISAPSVI LATGGLGHLY SATTNPAGST GDGIALGLWA GVAVSDLEFI QFHPTMLFAG 250 260 270 280 290 300 RAGGRRPLIT EAIRGEGAIL VDRQGNSITA GVHPMGDLAP RDVVAAAIDA RLKATGDPCV 310 320 330 340 350 360 YLDARGIEGF ASRFPTVTAS CRAAGIDPVR QPIPVVPGAH YSCGGIVTDV YGQTELLGLY 370 380 390 400 410 420 AAGEVARTGL HGANRLASNS LLEGLVVGGR AGKAAAAHAA AAGRSRATSS ATWPEPISYT 430 440 450 460 470 480 ALDRGDLQRA MSRDASMYRA AAGLHRLCDS LSGAQVRDVA CRRDFEDVAL TLVAQSVTAA 490 500 510 520 ALARTESRGC HHRAEYPCTV PEQARSIVVR GADDANAVCV QALVAVC

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842577 Genomic DNA. Translation: CAB09074.1. AE000516 Genomic DNA. Translation: AAK45899.1.
PIR	E70543.
RefSeq	NP_216111.1. NP_336085.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	886285. 924303.
GenomeReviews	Gene locus MT1631 in contig AE000516_GR. Gene locus Rv1595 in contig AL123456_GR.
KEGG	mtc:MT1631. mtu:Rv1595.
TIGR	MT1631.
Organism-specific databases
TubercuList	Rv1595.
Phylogenomic databases
HOGENOM	P65499.
OMA	GAYIWNR.
Enzyme and pathway databases
BRENDA	1.4.3.16. 809.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_MYCTU

Accession

Primary (citable) accession number: P65499
Secondary accession number(s): O06595

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)