Reviewed, UniProtKB/Swiss-Prot P65453 (MURA_BRUSU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase
    UDP-N-acetylglucosamine enolpyruvyl transferase
      Short name=EPT

Gene names

Name:	murA
Ordered Locus Names:	BR0254

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	UDP-N-acetylglucosamine 1-carboxyvinyltransferase HAMAP MF_00111		PRO_0000178852
Sites
	Active site	126	1	Proton donor By similarity
	Binding site	126	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65453-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5F38AF6BFC895CA7	FASTA	429	45,719
	10 20 30 40 50 60 MDRIKIVGGN KLNGVIPISG AKNAALPLMI ASLLTDDTLT LENVPHLADV EQLIRILSNH 70 80 90 100 110 120 GVDYSVNGRR EHQNGPYSRT IHFTARNIVD TTAPYELVSR MRASFWVIGP LLARMGEANV 130 140 150 160 170 180 SLPGGCAIGT RPVDLLLDAL LALGAEIDIE NGYAKAKARN GLVGARYKFP KVSVGATHVM 190 200 210 220 230 240 LMAATLAKGE TIIENAAREP EVANLADCLN AMGAKISGAG SSTIHVQGVT NLSGARVRII 250 260 270 280 290 300 PDRIEAGTYA MAVAMTGGDV LLEGAQESQL SCVLETLRQA GAEINETNSG LRVVRNGHGI 310 320 330 340 350 360 QPVDITTDPF PGFPTDLQAQ FMGLMTRAKG TSHITETIFE NRFMHVQELA RLGAKISLSG 370 380 390 400 410 420 QTATVEGVER LKGAQVMATD LRASVSLVIA GLAAEGETIV NRVYHLDRGF ERLEEKLSRC GADVKRISG

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29203.1.
RefSeq	NP_697288.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165913.
GenomeReviews	Gene locus BR0254 in contig AE014291_GR.
KEGG	bms:BR0254.
TIGR	BR0254.
Phylogenomic databases
HOGENOM	P65453.
OMA	VSRVYHL
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0254-MON.
BRENDA	2.5.1.7. 281610.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA_BRUSU

Accession

Primary (citable) accession number: P65453
Secondary accession number(s): Q8YF61

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 24, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)