Reviewed, UniProtKB/Swiss-Prot P65444 (MSAB2_STRR6)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA/msrB 2
Including the following 2 domains:
    1- Recommended name:
            Peptide methionine sulfoxide reductase msrA
                Short name=Protein-methionine-S-oxide reductase
              EC=1.8.4.11
        Alternative name(s):
            Peptide-methionine (S)-S-oxide reductase
              Short name=Peptide Met(O) reductase
    2- Recommended name:
            Peptide methionine sulfoxide reductase msrB
              EC=1.8.4.12
        Alternative name(s):
            Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrAB2
Synonyms:	msrA
Ordered Locus Names:	spr0577

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Complete proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity. HAMAP MF_01400
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin.
Sequence similarities	In the N-terminal section; belongs to the msrA Met sulfoxide reductase family. In the C-terminal section; belongs to the msrB Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Peptide methionine sulfoxide reductase msrA/msrB 2 HAMAP MF_01400		PRO_0000138520
Regions
	Region	1 – 155	155	Peptide methionine sulfoxide reductase A HAMAP MF_01400
	Region	172 – 295	124	Peptide methionine sulfoxide reductase B HAMAP MF_01400
Sites
	Active site	10	1	By similarity
	Active site	284	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65444-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 712B56F23489A40E	FASTA	311	35,610
	10 20 30 40 50 60 MHEIYLAGGC FWGVEEYFSR VPGVTDAVSG YANGRGETTK YELINQTGHA ETVHVTYDAK 70 80 90 100 110 120 QISLKEILLH YFRIINPTSK NKQGNDVGTQ YRTGVYYTDD KDLEVINQVF DEVAKKYDQP 130 140 150 160 170 180 LAVEKENLKN FVVAEDYHQD YLKKNPNGYC HINVNQAAYP VIDASKYPKP SDEELKKTLS 190 200 210 220 230 240 PEEYAVTQEN QTERAFSNRY WDKFESGIYV DIATGEPLFS SKDKFESGCG WPSFTQPISP 250 260 270 280 290 300 DVVTYKEDKS YNMTRMEVRS RVGDSHLGHV FTDGPQDKGG LRYCINSLSI RFIPKDQMEE 310 KGYAYLLDYV D

References

[1]

"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed: 11544234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007317 Genomic DNA. Translation: AAK99381.1. Different initiation.
PIR	A97944.
RefSeq	NP_358171.1.
3D structure databases
SMR	P65444. Positions 4-161, 164-306.
ModBase	Search...
Protein-protein interaction databases
STRING	P65444.
Genome annotation databases
GeneID	933291.
GenomeReviews	Gene locus spr0577 in contig AE007317_GR.
KEGG	spr:spr0577.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0225.
HOGENOM	HBG715255.
PhylomeDB	P65444.
Enzyme and pathway databases
BioCyc	SPNE171101:SPR0577-MONOMER.
Family and domain databases
HAMAP	MF_01400. MsrB. Fused. [Tree] MF_01401. MsrA. Fused. [Tree]
InterPro	IPR002579. Methionine_sulphoxide_MsrB. IPR011057. Mss4-like. IPR002569. Peptide_Met_Sox_Rdtase_MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit. G3DSA:2.170.150.20. MsrB. 1 hit.
Pfam	PF01625. PMSR. 1 hit. PF01641. SelR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00401. msrA. 1 hit. TIGR00357. MsrB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSAB2_STRR6

Accession

Primary (citable) accession number: P65444
Secondary accession number(s): Q97RX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)