P65429 (RSMH_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Rv2165c, MT2223
ORF Names:	MTCY270.03

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_01007
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.
Sequence caution	The sequence CAB08662.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108667
Regions
	Region	49 – 51	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	68	1	S-adenosyl-L-methionine By similarity
	Binding site	95	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	119	1	S-adenosyl-L-methionine By similarity
	Binding site	126	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65429 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 4B46C1C76EB4538E	FASTA	344	37,152
	10 20 30 40 50 60 MADPGSGPTG FGHVPVLAQR CFELLTPALT RYYPDGSQAV LLDATIGAGG HAERFLEGLP 70 80 90 100 110 120 GLRLIGLDRD PTALDVARSR LVRFADRLTL VHTRYDCLGA ALAESGYAAV GSVDGILFDL 130 140 150 160 170 180 GVSSMQLDRA ERGFAYATDA PLDMRMDPTT PLTAADIVNT YDEAALADIL RRYGEERFAR 190 200 210 220 230 240 RIAAGIVRRR AKTPFTSTAE LVALLYQAIP APARRVGGHP AKRTFQALRI AVNDELESLR 250 260 270 280 290 300 TAVPAALDAL AIGGRIAVLA YQSLEDRIVK RVFAEAVASA TPAGLPVELP GHEPRFRSLT 310 320 330 340 HGAERASVAE IERNPRSTPV RLRALQRVEH RAQSQQWATE KGDS

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842579 Genomic DNA. Translation: CAB08662.1. Different initiation. AE000516 Genomic DNA. Translation: AAK46508.1.
PIR	A70581.
RefSeq	NP_336694.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P65429.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000002259; EBMYCP00000002259; EBMYCG00000002257. EBMYCT00000069798; EBMYCP00000067857; EBMYCG00000069793.
GeneID	924236.
GenomeReviews	Gene locus MT2223 in contig AE000516_GR. Gene locus Rv2165c in contig AL123456_GR.
KEGG	mtc:MT2223. mtu:Rv2165c.
PATRIC	18126646. VBIMycTub22151_2435.
TIGR	MT2223.
Organism-specific databases
TubercuList	Rv2165c.
Phylogenomic databases
GeneTree	EBGT00050000016736.
HOGENOM	HBG302779.
OMA	NPATRAF.
PhylomeDB	P65429.
ProtClustDB	PRK00050.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_MYCTU

Accession

Primary (citable) accession number: P65429
Secondary accession number(s): O06212

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program