P65425 (MENC_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable o-succinylbenzoate synthase
Short name=OSB synthase
Short name=OSBS
EC=4.2.1.113

Alternative name(s):
4-(2'-carboxyphenyl)-4-oxybutyric acid synthase
o-succinylbenzoic acid synthase

Gene names

Name:	menC
Ordered Locus Names:	Rv0553, MT0579
ORF Names:	MTCY25D10.32

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts SHCHC to OSB By similarity. HAMAP MF_00470
Catalytic activity	(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O. HAMAP MF_00470
Cofactor	Magnesium By similarity. HAMAP MF_00470
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 4/8. HAMAP MF_00470
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00470
Sequence similarities	Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 1 subfamily.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydro-lyase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Probable o-succinylbenzoate synthase HAMAP MF_00470		PRO_0000171279
Sites
	Active site	110	1	By similarity
	Active site	212	1	By similarity
	Metal binding	138	1	Magnesium By similarity
	Metal binding	165	1	Magnesium By similarity
	Metal binding	188	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65425 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 422999B373336667	FASTA	326	33,975
	10 20 30 40 50 60 MIPVLPPLEA LLDRLYVVAL PMRVRFRGIT TREVALIEGP AGWGEFGAFV EYQSAQACAW 70 80 90 100 110 120 LASAIETAYC APPPVRRDRV PINATVPAVA AAQVGEVLAR FPGARTAKVK VAEPGQSLAD 130 140 150 160 170 180 DIERVNAVRE LVPMVRVDAN GGWGVAEAVA AAAALTADGP LEYLEQPCAT VAELAELRRR 190 200 210 220 230 240 VDVPIAADES IRKAEDPLAV VRAQAADIAV LKVAPLGGIS ALLDIAARIA VPVVVSSALD 250 260 270 280 290 300 SAVGIAAGLT AAAALPELDH ACGLGTGGLF EEDVAEPAAP VDGFLAVART TPDPARLQAL 310 320 GAPPQRRQWW IDRVKACYSL LVPSFG

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842573 Genomic DNA. Translation: CAB08964.1. AE000516 Genomic DNA. Translation: AAK44802.1.
PIR	D70548.
RefSeq	NP_215067.1. NC_000962.2. NP_334988.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P65425.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000653; EBMYCP00000000653; EBMYCG00000000653. EBMYCT00000069772; EBMYCP00000067831; EBMYCG00000069767.
GeneID	887544. 924942.
GenomeReviews	Gene locus MT0579 in contig AE000516_GR. Gene locus Rv0553 in contig AL123456_GR.
KEGG	mtc:MT0579. mtu:Rv0553.
PATRIC	18122974. VBIMycTub22151_0621.
TIGR	MT0579.
Organism-specific databases
TubercuList	Rv0553.
Phylogenomic databases
GeneTree	EBGT00050000017426.
HOGENOM	HBG568083.
OMA	LEYVEQP.
ProtClustDB	PRK02901.
Family and domain databases
HAMAP	MF_00470. MenC_1. [Tree]
InterPro	IPR013342. Mandelate_racemase_C. IPR001354. MR_MLE. IPR010196. OSB_synthase. [Graphical view]
KO	K02549.
PANTHER	PTHR13794. MR_MLE. 1 hit.
Pfam	PF01188. MR_MLE. 1 hit. [Graphical view]
SMART	SM00922. MR_MLE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MENC_MYCTU

Accession

Primary (citable) accession number: P65425
Secondary accession number(s): O06419

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program