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P65396 (MOAE_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Molybdopterin synthase catalytic subunit

EC=2.8.1.12
Alternative name(s):
MPT synthase subunit 2
Molybdenum cofactor biosynthesis protein E
Molybdopterin-converting factor large subunit
Molybdopterin-converting factor subunit 2
Gene names
Name:moaE
Ordered Locus Names:BMEI1254
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length163 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by MoaD By similarity.

Catalytic activity

Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H2O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein].

Pathway

Cofactor biosynthesis; molybdopterin biosynthesis.

Subunit structure

Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Also stable as homodimer. The enzyme changes between these two forms during catalysis By similarity.

Sequence similarities

Belongs to the MoaE family.

Ontologies

Keywords
   Biological processMolybdenum cofactor biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processMo-molybdopterin cofactor biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionmolybdopterin synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 163163Molybdopterin synthase catalytic subunit
PRO_0000163079

Regions

Region43 – 453Substrate binding By similarity
Region107 – 1082Substrate binding By similarity
Region130 – 1323Substrate binding By similarity

Sites

Binding site1231Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P65396 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: B622143ED5116118

FASTA16318,396
        10         20         30         40         50         60 
MDGQPTCPLS ISVRSEDFDI AAEIDRIGKN RREIGAIVTF TGLCRDEDGQ LAALELEHYP 

        70         80         90        100        110        120 
GMAQAEISRM AKQALERWPL NGLTIIHRYG LIKPGENIVL VVAASRHRHA AFEAASFLMD 

       130        140        150        160 
YMKTNAPFWK REHLADGTAG GWVSSKEEDE ESRKRWEEPR KSE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52435.1.
PIRAH3408.
RefSeqNP_540171.1. NC_003317.1.

3D structure databases

ProteinModelPortalP65396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52435; AAL52435; BMEI1254.
GeneID1196965.
KEGGbme:BMEI1254.
PATRIC17797467. VBIBruMel146950_0552.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000280876.
KOK03635.
OMARERWPLN.
OrthoDBEOG6KMBD9.
ProtClustDBCLSK898364.

Enzyme and pathway databases

UniPathwayUPA00344.

Family and domain databases

Gene3D3.90.1170.40. 1 hit.
InterProIPR003448. Mopterin_biosynth_MoaE.
[Graphical view]
PANTHERPTHR23404:SF2. PTHR23404:SF2. 1 hit.
PfamPF02391. MoaE. 1 hit.
[Graphical view]
SUPFAMSSF54690. SSF54690. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMOAE_BRUME
AccessionPrimary (citable) accession number: P65396
Secondary accession number(s): Q8YGA6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names