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Protein

Cyclic pyranopterin monophosphate synthase

Gene

moaA

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).By similarity

Catalytic activityi

GTP = cyclic pyranopterin phosphate + diphosphate.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.By similarity
  • S-adenosyl-L-methionineBy similarityNote: Binds 1 S-adenosyl-L-methionine per subunit.By similarity

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171GTPBy similarity
Metal bindingi24 – 241Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi28 – 281Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Binding sitei30 – 301S-adenosyl-L-methionineBy similarity
Metal bindingi31 – 311Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Binding sitei71 – 711GTPBy similarity
Binding sitei75 – 751S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei102 – 1021GTPBy similarity
Binding sitei126 – 1261S-adenosyl-L-methionineBy similarity
Binding sitei163 – 1631GTPBy similarity
Binding sitei197 – 1971S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
Metal bindingi261 – 2611Iron-sulfur 2 (4Fe-4S-substrate)By similarity
Metal bindingi264 – 2641Iron-sulfur 2 (4Fe-4S-substrate)By similarity
Metal bindingi278 – 2781Iron-sulfur 2 (4Fe-4S-substrate)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-2218-MONOMER.
BRENDAi4.1.99.18. 3352.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic pyranopterin monophosphate synthase (EC:4.1.99.18)
Alternative name(s):
Molybdenum cofactor biosynthesis protein A
Gene namesi
Name:moaA
Ordered Locus Names:SA2063
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Cyclic pyranopterin monophosphate synthasePRO_0000152992Add
BLAST

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP65388.
SMRiP65388. Positions 3-329.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP65388.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 2683GTP bindingBy similarity

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.Curated

Phylogenomic databases

HOGENOMiHOG000228681.
OMAiGWIHQIR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65388-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEQIKDKLG RPIRDLRLSV TDRCNFRCDY CMPKEVFGDD FVFLPKNELL
60 70 80 90 100
TFDEMARIAK VYAELGVKKI RITGGEPLMR RDLDVLIAKL NQIDGIEDIG
110 120 130 140 150
LTTNGLLLKK HGQKLYDAGL RRINVSLDAI DDTLFQSINN RNIKATTILE
160 170 180 190 200
QIDYATSIGL NVKVNVVIQK GINDDQIIPM LEYFKDKHIE IRFIEFMDVG
210 220 230 240 250
NDNGWDFSKV VTKDEMLTMI EQHFEIDPVE PKYFGEVAKY YRHKDNGVQF
260 270 280 290 300
GLITSVSQSF CSTCTRARLS SDGKFYGCLF ATVDGFNVKA FIRSGVTDEE
310 320 330 340
LKEQFKALWQ IRDDRYSDER TAQTVANRQR KKINMNYIGG
Length:340
Mass (Da):39,078
Last modified:October 11, 2004 - v1
Checksum:iBDCDFE1014E98306
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43360.1.
PIRiG90024.
RefSeqiWP_000230173.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB43360; BAB43360; BAB43360.
PATRICi19576640. VBIStaAur116463_2238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43360.1.
PIRiG90024.
RefSeqiWP_000230173.1. NC_002745.2.

3D structure databases

ProteinModelPortaliP65388.
SMRiP65388. Positions 3-329.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB43360; BAB43360; BAB43360.
PATRICi19576640. VBIStaAur116463_2238.

Phylogenomic databases

HOGENOMiHOG000228681.
OMAiGWIHQIR.

Enzyme and pathway databases

UniPathwayiUPA00344.
BioCyciSAUR158879:GJCB-2218-MONOMER.
BRENDAi4.1.99.18. 3352.

Miscellaneous databases

EvolutionaryTraceiP65388.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_STAAN
AccessioniPrimary (citable) accession number: P65388
Secondary accession number(s): Q99S04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.