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Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17GTPUniRule annotation1
Metal bindingi24Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi28Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei30S-adenosyl-L-methionineUniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei71GTPUniRule annotation1
Binding sitei75S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei102GTPUniRule annotation1
Binding sitei126S-adenosyl-L-methionineUniRule annotation1
Binding sitei163GTPUniRule annotation1
Binding sitei197S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi261Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi264Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi278Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi266 – 268GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi4.1.99.18. 3352.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:SA2063
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001529921 – 340GTP 3',8-cyclaseAdd BLAST340

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP65388.
SMRiP65388.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP65388.

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228681.
KOiK03639.
OMAiGWIHQIR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65388-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEQIKDKLG RPIRDLRLSV TDRCNFRCDY CMPKEVFGDD FVFLPKNELL
60 70 80 90 100
TFDEMARIAK VYAELGVKKI RITGGEPLMR RDLDVLIAKL NQIDGIEDIG
110 120 130 140 150
LTTNGLLLKK HGQKLYDAGL RRINVSLDAI DDTLFQSINN RNIKATTILE
160 170 180 190 200
QIDYATSIGL NVKVNVVIQK GINDDQIIPM LEYFKDKHIE IRFIEFMDVG
210 220 230 240 250
NDNGWDFSKV VTKDEMLTMI EQHFEIDPVE PKYFGEVAKY YRHKDNGVQF
260 270 280 290 300
GLITSVSQSF CSTCTRARLS SDGKFYGCLF ATVDGFNVKA FIRSGVTDEE
310 320 330 340
LKEQFKALWQ IRDDRYSDER TAQTVANRQR KKINMNYIGG
Length:340
Mass (Da):39,078
Last modified:October 11, 2004 - v1
Checksum:iBDCDFE1014E98306
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43360.1.
PIRiG90024.
RefSeqiWP_000230173.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB43360; BAB43360; BAB43360.
GeneIDi28380127.
KEGGisau:SA2063.
PATRICi19576640. VBIStaAur116463_2238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43360.1.
PIRiG90024.
RefSeqiWP_000230173.1. NC_002745.2.

3D structure databases

ProteinModelPortaliP65388.
SMRiP65388.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB43360; BAB43360; BAB43360.
GeneIDi28380127.
KEGGisau:SA2063.
PATRICi19576640. VBIStaAur116463_2238.

Phylogenomic databases

HOGENOMiHOG000228681.
KOiK03639.
OMAiGWIHQIR.

Enzyme and pathway databases

UniPathwayiUPA00344.
BRENDAi4.1.99.18. 3352.

Miscellaneous databases

EvolutionaryTraceiP65388.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_STAAN
AccessioniPrimary (citable) accession number: P65388
Secondary accession number(s): Q99S04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.