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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 208ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-1210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:MW1186
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311tRNA dimethylallyltransferasePRO_0000163977Add
BLAST

Proteomic databases

PRIDEiP65356.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei104 – 1041Interaction with substrate tRNAUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP65356.
SMRiP65356. Positions 5-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 206Substrate bindingUniRule annotation
Regioni38 – 414Interaction with substrate tRNAUniRule annotation
Regioni166 – 1705Interaction with substrate tRNAUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000039995.
OMAiHTRNYAK.
OrthoDBiEOG661HB3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

P65356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKNKPFIVV IVGPTASGKT ELSIELAKRI NGEIISGDSM QVYKHMNIGT
60 70 80 90 100
AKVTPEEMDG IPHHLIDILN PDDTFSAYEF KRLAEDLITD ITNRGKVPII
110 120 130 140 150
AGGTGLYIQS LIYNYELEDE TVTPAQLSIV KQKLSALEHL DNQQLHDYLA
160 170 180 190 200
QFDAVSAENI HPNNRQRVLR AIEYYLKTKK LLSNRKKVQQ FTENYDTLLL
210 220 230 240 250
GIEMSRKTLY SRINKRVDIM LDHGLFREVQ QLVEQGYESC QSMQAIGYKE
260 270 280 290 300
LIPVINGQMI YEDAVNDLKQ HSRQYAKRQM TWFKNKMSVH WLDKENMSLQ
310
MMLDEITTQI K
Length:311
Mass (Da):35,868
Last modified:October 11, 2004 - v1
Checksum:i6011E9D44878043E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB95051.1.

Genome annotation databases

EnsemblBacteriaiBAB95051; BAB95051; BAB95051.
PATRICi19568968. VBIStaAur44266_1245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB95051.1.

3D structure databases

ProteinModelPortaliP65356.
SMRiP65356. Positions 5-310.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP65356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB95051; BAB95051; BAB95051.
PATRICi19568968. VBIStaAur44266_1245.

Phylogenomic databases

HOGENOMiHOG000039995.
OMAiHTRNYAK.
OrthoDBiEOG661HB3.

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-1210-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MW2.

Entry informationi

Entry nameiMIAA_STAAW
AccessioniPrimary (citable) accession number: P65356
Secondary accession number(s): Q99UH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 16, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.