P65328 (RISA_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase
Short name=RS
EC=2.5.1.9

Gene names

Name:	ribE
Synonyms:	ribC
Ordered Locus Names:	Mb1447

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Homotrimer By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Riboflavin synthase		PRO_0000068169
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 197	100	Lumazine-binding 2
	Region	4 – 6	3	Substrate binding By similarity
	Region	47 – 49	3	Substrate binding By similarity
	Region	62 – 67	6	Substrate binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65328 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 1CE1C3A20A61D2FF	FASTA	201	21,320
	10 20 30 40 50 60 MFTGIVEERG EVTGREALVD AARLTIRGPM VTADAGHGDS IAVNGVCLTV VDVLPDGQFT 70 80 90 100 110 120 ADVMAETLNR SNLGELRPGS RVNLERAAAL GSRLGGHIVQ GHVDATGEIV ARCPSEHWEV 130 140 150 160 170 180 VRIEMPASVA RYVVEKGSIT VDGISLTVSG LGAEQRDWFE VSLIPTTREL TTLGSAAVGT 190 200 RVNLEVDVVA KYVERLMRSA G

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248338 Genomic DNA. Translation: CAD94308.1.
RefSeq	NP_855099.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P65328.
SMR	P65328. Positions 1-199.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb1447.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD94308; CAD94308; Mb1447.
GeneID	1092304.
KEGG	mbo:Mb1447.
PATRIC	18004870. VBIMycBov88188_1582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0307.
HOGENOM	HOG000151758.
KO	K00793.
OMA	SRYVVEK.
ProtClustDB	PRK09289.
Enzyme and pathway databases
UniPathway	UPA00275; UER00405.
Family and domain databases
Gene3D	2.40.30.20. 2 hits.
InterPro	IPR023366. ATPase_F1/A1-cplx_a_su_N. IPR001783. Lumazine-bd. IPR026017. Lumazine-bd_dom. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR21098. PTHR21098. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_MYCBO

Accession

Primary (citable) accession number: P65328
Secondary accession number(s): P71680

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program