P65327 (RISA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase alpha chain
EC=2.5.1.9

Gene names

Name:	ribE
Synonyms:	ribC
Ordered Locus Names:	Rv1412, MT1456
ORF Names:	MTCY21B4.29

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from direct assay. Source: MTBBASE
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from direct assay. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Riboflavin synthase alpha chain		PRO_0000068168
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 197	100	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	P65327 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 1CE1C3A20A61D2FF	FASTA	201	21,320
	10 20 30 40 50 60 MFTGIVEERG EVTGREALVD AARLTIRGPM VTADAGHGDS IAVNGVCLTV VDVLPDGQFT 70 80 90 100 110 120 ADVMAETLNR SNLGELRPGS RVNLERAAAL GSRLGGHIVQ GHVDATGEIV ARCPSEHWEV 130 140 150 160 170 180 VRIEMPASVA RYVVEKGSIT VDGISLTVSG LGAEQRDWFE VSLIPTTREL TTLGSAAVGT 190 200 RVNLEVDVVA KYVERLMRSA G

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842576 Genomic DNA. Translation: CAB02162.1. AE000516 Genomic DNA. Translation: AAK45721.1.
PIR	A70902.
RefSeq	NP_215928.1. NC_000962.2. NP_335907.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P65327.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001303; EBMYCP00000001303; EBMYCG00000001303. EBMYCT00000070551; EBMYCP00000068610; EBMYCG00000070546.
GeneID	886690. 924516.
GenomeReviews	Gene locus MT1456 in contig AE000516_GR. Gene locus Rv1412 in contig AL123456_GR.
KEGG	mtc:MT1456. mtu:Rv1412.
PATRIC	18124970. VBIMycTub22151_1599.
TIGR	MT1456.
Organism-specific databases
TubercuList	Rv1412.
Phylogenomic databases
GeneTree	EBGT00050000017347.
HOGENOM	HBG289809.
OMA	PVNLEVD.
PhylomeDB	P65327.
ProtClustDB	PRK09289.
Family and domain databases
InterPro	IPR023366. ATPase_F1/A1-cplx_a_su_N. IPR001783. Lumazine-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:2.40.30.20. G3DSA:2.40.30.20. 2 hits.
KO	K00793.
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.
TIGRFAMs	TIGR00187. RibE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_MYCTU

Accession

Primary (citable) accession number: P65327
Secondary accession number(s): P71680

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program