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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (BMEI0835)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 61ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:BMEII1028
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001909141 – 341Tetraacyldisaccharide 4'-kinaseAdd BLAST341

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_1573

Structurei

3D structure databases

ProteinModelPortaliP65325
SMRiP65325
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD Bacteria
COG1663 LUCA
HOGENOMiHOG000004954
KOiK00912
OMAiMDDGFQN

Family and domain databases

HAMAPiMF_00409 LpxK, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR003758 Tetraacyldisaccharide_4-kinase
PANTHERiPTHR42724 PTHR42724, 1 hit
PfamiView protein in Pfam
PF02606 LpxK, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00682 lpxK, 1 hit

Sequencei

Sequence statusi: Complete.

P65325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEAPPFWW DEPDWRALAL APAAWIYGRV SGRRLIRAVP PRVSLPVLCV
60 70 80 90 100
GNFTVGGAGK TPTAIAFARG AIARGMKPGI VSRGYGGNYS GLHLVDPGHD
110 120 130 140 150
GARHVGDEPL LLARHAAVAL SPDRVKAAEY LKSLGCDFII MDDGFQSARL
160 170 180 190 200
HADFSLLVVD ASRGIGNGRV IPAGPLRAPL TDQMRKTDAL LCIGKGNGAD
210 220 230 240 250
FVIRQAARAG RPIYHAQLRP SSSATVAGRR WLAFAGIGNP DKFYESVRQA
260 270 280 290 300
GGEVVETHSF ADHYSFEPDD IRGLVDMARR QGLGLITTAK DHVRLATMPG
310 320 330 340
VPPEFLSKLA VLDVDLEFDR TDALDHILDT VVERFKSRLH G
Length:341
Mass (Da):36,782
Last modified:October 11, 2004 - v1
Checksum:i93014F0F6B7C3DCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008918 Genomic DNA Translation: AAL54270.1
PIRiAC3638
RefSeqiWP_002966366.1, NZ_GG703779.1

Genome annotation databases

EnsemblBacteriaiAAL54270; AAL54270; BMEII1028
GeneIDi29595742
KEGGibme:BMEII1028
bmel:DK63_2228
PATRICifig|224914.52.peg.2334

Similar proteinsi

Entry informationi

Entry nameiLPXK_BRUME
AccessioniPrimary (citable) accession number: P65325
Secondary accession number(s): Q8YB72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome