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P65325 (LPXK_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tetraacyldisaccharide 4'-kinase

EC=2.7.1.130
Alternative name(s):
Lipid A 4'-kinase
Gene names
Name:lpxK
Ordered Locus Names:BMEII1028
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP-Rule MF_00409

Catalytic activity

ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP-Rule MF_00409

Pathway

Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP-Rule MF_00409

Sequence similarities

Belongs to the LpxK family.

Ontologies

Keywords
   Biological processLipid A biosynthesis
Lipid biosynthesis
Lipid metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlipid A biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tetraacyldisaccharide 4'-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Tetraacyldisaccharide 4'-kinase HAMAP-Rule MF_00409
PRO_0000190914

Regions

Nucleotide binding54 – 618ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P65325 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 93014F0F6B7C3DCE

FASTA34136,782
        10         20         30         40         50         60 
MASEAPPFWW DEPDWRALAL APAAWIYGRV SGRRLIRAVP PRVSLPVLCV GNFTVGGAGK 

        70         80         90        100        110        120 
TPTAIAFARG AIARGMKPGI VSRGYGGNYS GLHLVDPGHD GARHVGDEPL LLARHAAVAL 

       130        140        150        160        170        180 
SPDRVKAAEY LKSLGCDFII MDDGFQSARL HADFSLLVVD ASRGIGNGRV IPAGPLRAPL 

       190        200        210        220        230        240 
TDQMRKTDAL LCIGKGNGAD FVIRQAARAG RPIYHAQLRP SSSATVAGRR WLAFAGIGNP 

       250        260        270        280        290        300 
DKFYESVRQA GGEVVETHSF ADHYSFEPDD IRGLVDMARR QGLGLITTAK DHVRLATMPG 

       310        320        330        340 
VPPEFLSKLA VLDVDLEFDR TDALDHILDT VVERFKSRLH G 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008918 Genomic DNA. Translation: AAL54270.1.
PIRAC3638.
RefSeqNP_542006.1. NC_003318.1.

3D structure databases

ProteinModelPortalP65325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEII1028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL54270; AAL54270; BMEII1028.
GeneID1198800.
KEGGbme:BMEII1028.
PATRIC17798931. VBIBruMel146950_1809.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000004954.
KOK00912.
OMAPKRADAV.
OrthoDBEOG60GRWT.

Enzyme and pathway databases

UniPathwayUPA00359; UER00482.

Family and domain databases

HAMAPMF_00409. LpxK.
InterProIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00682. lpxK. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLPXK_BRUME
AccessionPrimary (citable) accession number: P65325
Secondary accession number(s): Q8YB72
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names