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Reviewed, UniProtKB/Swiss-Prot P65320 (LPXA_BRUME)

Last modified February 9, 2010. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
      Short name=UDP-N-acetylglucosamine acyltransferase
    EC=2.3.1.129
Gene names
Name: lpxA
Ordered Locus Names: BMEI0833
OrganismBrucella melitensis [Complete proteome] [HAMAP]
Taxonomic identifier29459 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00387

Catalytic activity

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387

Pathway

Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387

Subunit structure

Homotrimer By similarity. HAMAP MF_00387

Subcellular location

Cytoplasm By similarity HAMAP MF_00387.

Sequence similarities

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
   Biological processLipid A biosynthesis
Lipid synthesis
   Cellular componentCytoplasm
   DomainRepeat
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processlipid A biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionacyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387
PRO_0000188036

Sequences

Sequence LengthMass (Da)Tools
P65320-1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: DF77C706EEA9F8DF

FASTA27829,386
        10         20         30         40         50         60 
MKETFIHPTA LVEPGVELGQ GVSVGPFCHV QSGAIIGNDC ELMSHVVITG ATTLGAGTKV 

        70         80         90        100        110        120 
YPHAILGCDP QNNKHKGGPT RLNVGVNCII REGVTMHKGS DNARGYTSIG DNCSFLAYAH 

       130        140        150        160        170        180 
VAHDCDIGDY VTFSNNVMIG GHTSIGHHAI LGGGAAVHQF VRVGHHAFIG GLAAVVSDLI 

       190        200        210        220        230        240 
PYGMAIGVHA HLGGLNIIGM KRSGMERKEI HNLRHAVRML FDRTKPIRQR AQDVLAAIPD 

       250        260        270 
SPTVSDMISF INVDTKRAYC TPPLDAAHGG AGHDSDED 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52014.1. Different initiation.
PIRAC3356.
RefSeqNP_539750.1.

3D structure databases

SMRP65320. Positions 2-260.
ModBaseSearch...

Genome annotation databases

GeneID1196544.
GenomeReviewsGene locus BMEI0833 in contig AE008917_GR.
KEGGbme:BMEI0833.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG659295.
OMADLKYQGE.
PhylomeDBP65320.

Enzyme and pathway databases

BioCycBMEL224914:BMEI0833-MONOMER.
BRENDA2.3.1.129. 277959.

Family and domain databases

HAMAPMF_00387. LpxA.
[Tree]
InterProIPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_lpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PIRSFPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
TIGRFAMsTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLPXA_BRUME
AccessionPrimary (citable) accession number: P65320
Secondary accession number(s): Q8YHG8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents