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P65258 (LDH2_STAAM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-lactate dehydrogenase 2
Short name=L-LDH 2
EC=1.1.1.27
Gene names
Name:ldhB
Ordered Locus Names:SAV2602
OrganismStaphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]
Taxonomic identifier158878 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells, despite playing a secondary role in this resistance mechanism By similarity. HAMAP MF_00488

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488

Subunit structure

Homotetramer By similarity. HAMAP MF_00488

Subcellular location

Cytoplasm By similarity HAMAP MF_00488.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
   Biological processGlycolysis
Stress response
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319L-lactate dehydrogenase 2 HAMAP MF_00488
PRO_0000168388

Regions

Nucleotide binding14 – 4229NAD By similarity

Sites

Active site1781Proton acceptor By similarity
Binding site911Substrate By similarity
Binding site1231NAD or substrate By similarity
Binding site1541Substrate By similarity
Binding site2311Substrate By similarity

Amino acid modifications

Modified residue2221Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
P65258 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 7779343BCA801CED

FASTA31934,432
        10         20         30         40         50         60 
MKTFGKKVVL IGDGSVGSSY AFAMVTQGVA DEFVIIDIAK DKVKADVQDL NHGTVHSPSP 

        70         80         90        100        110        120 
VDVKAGEYED CKDADLVVIT AGAPQKPGET RLQLVEKNTK IMKSIVKSVM DSGFDGYFLI 

       130        140        150        160        170        180 
AANPVDILTR FVKEYTGLPA ERVIGSGTVL DSARLQYLIS QELGVAPSSV DASIIGEHGD 

       190        200        210        220        230        240 
TELAVWSQAN VAGISVYDTL KEQTGSEAKA EEIYVNTRDA AYEIIQAKGS TYYGIALALM 

       250        260        270        280        290        300 
RISKAILNNE NNVLNVSIQL DGQYGGHKGV YLGVPTLVNQ HGAVKIYEMP LSAEEQALFD 

       310 
KSVKILEDTF DSIKYLLED

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000017 Genomic DNA. Translation: BAB58764.1.
RefSeqNP_373126.1. NC_002758.2.

3D structure databases

ProteinModelPortalP65258.
SMRP65258. Positions 5-313.
ModBaseSearch...

Protein-protein interaction databases

STRINGP65258.

2D gel databases

World-2DPAGE0002:P65258.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000007413; EBSTAP00000007231; EBSTAG00000007412.
GeneID1122627.
GenomeReviewsGene locus SAV2602 in contig BA000017_GR.
KEGGsav:SAV2602.
PATRIC19566140. VBIStaAur52173_2696.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
GeneTreeEBGT00050000024431.
HOGENOMHBG566126.
OMAIHPLSCH.
PhylomeDBP65258.
ProtClustDBCLSK886101.

Enzyme and pathway databases

BioCycSAUR158878:SAV2602-MONOMER.

Family and domain databases

HAMAPMF_00488. Lactate_dehydrog.
[Tree]
InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00016.
PANTHERPTHR11540:SF3. PTHR11540:SF3. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLDH2_STAAM
AccessionPrimary (citable) accession number: P65258
Secondary accession number(s): Q99R35
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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